Differential inhibition of the t cell activation pathway by dexamethasone and cyclosporine

Masutaka Furue, Yuko Kawakami, Toshiaki Kawakami, Stephen I. Katz

研究成果: ジャーナルへの寄稿記事

17 引用 (Scopus)

抄録

We examined the inhibitory capacity of dexamethasone (DEX) and cyclosporine (CsA) on T cell activation using various accessory cell (AC)-dependent and AC- independent stimuli. We found that CsA strongly inhibited T cell activation in each of the assays used: allogeneic T cell stimulation, phorbol myristate acetate plus concanavalin A, PMA plus anti-CD3 monoclonal antibody (2C11), or PMA plus ionomycin (IONO) T cell activation. DEX was a potent inhibitor of allogeneic stimulation and of the PMA+Con A- or PMA+2C11- induced T cell stimulation. PMA+IONO stimulation, however, was not affected by DEX. When inhibition occurred, both drugs suppressed [3H]TdR incorporation, IL-2 production, and IL-2 mRNA accumulation, indicating that the sites of interference of these drugs in the T cell activation pathway are located proximal to IL-2 mRNA accumulation. However, the difference in the effects of DEX and CsA in PMA+IONO stimulation suggests that DEX and CsA differentially affect T cell activation.

元の言語英語
ページ(範囲)560-564
ページ数5
ジャーナルTransplantation
49
発行部数3
DOI
出版物ステータス出版済み - 1 1 1990
外部発表Yes

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Dexamethasone
Cyclosporine
T-Lymphocytes
Ionomycin
Interleukin-2
Messenger RNA
Tetradecanoylphorbol Acetate
Concanavalin A
Pharmaceutical Preparations
Monoclonal Antibodies

All Science Journal Classification (ASJC) codes

  • Transplantation

これを引用

Differential inhibition of the t cell activation pathway by dexamethasone and cyclosporine. / Furue, Masutaka; Kawakami, Yuko; Kawakami, Toshiaki; Katz, Stephen I.

:: Transplantation, 巻 49, 番号 3, 01.01.1990, p. 560-564.

研究成果: ジャーナルへの寄稿記事

Furue, Masutaka ; Kawakami, Yuko ; Kawakami, Toshiaki ; Katz, Stephen I. / Differential inhibition of the t cell activation pathway by dexamethasone and cyclosporine. :: Transplantation. 1990 ; 巻 49, 番号 3. pp. 560-564.
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N2 - We examined the inhibitory capacity of dexamethasone (DEX) and cyclosporine (CsA) on T cell activation using various accessory cell (AC)-dependent and AC- independent stimuli. We found that CsA strongly inhibited T cell activation in each of the assays used: allogeneic T cell stimulation, phorbol myristate acetate plus concanavalin A, PMA plus anti-CD3 monoclonal antibody (2C11), or PMA plus ionomycin (IONO) T cell activation. DEX was a potent inhibitor of allogeneic stimulation and of the PMA+Con A- or PMA+2C11- induced T cell stimulation. PMA+IONO stimulation, however, was not affected by DEX. When inhibition occurred, both drugs suppressed [3H]TdR incorporation, IL-2 production, and IL-2 mRNA accumulation, indicating that the sites of interference of these drugs in the T cell activation pathway are located proximal to IL-2 mRNA accumulation. However, the difference in the effects of DEX and CsA in PMA+IONO stimulation suggests that DEX and CsA differentially affect T cell activation.

AB - We examined the inhibitory capacity of dexamethasone (DEX) and cyclosporine (CsA) on T cell activation using various accessory cell (AC)-dependent and AC- independent stimuli. We found that CsA strongly inhibited T cell activation in each of the assays used: allogeneic T cell stimulation, phorbol myristate acetate plus concanavalin A, PMA plus anti-CD3 monoclonal antibody (2C11), or PMA plus ionomycin (IONO) T cell activation. DEX was a potent inhibitor of allogeneic stimulation and of the PMA+Con A- or PMA+2C11- induced T cell stimulation. PMA+IONO stimulation, however, was not affected by DEX. When inhibition occurred, both drugs suppressed [3H]TdR incorporation, IL-2 production, and IL-2 mRNA accumulation, indicating that the sites of interference of these drugs in the T cell activation pathway are located proximal to IL-2 mRNA accumulation. However, the difference in the effects of DEX and CsA in PMA+IONO stimulation suggests that DEX and CsA differentially affect T cell activation.

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