Differential localization and regulation of stargazin-like protein, γ-8 and stargazin in the plasma membrane of hippocampal and cortical neurons

Mihoko Inamura, Makoto Itakura, Hirotsugu Okamoto, Sumio Hoka, Akira Mizoguchi, Yugo Fukazawa, Ryuichi Shigemoto, Saori Yamamori, Masami Takahashi

研究成果: ジャーナルへの寄稿記事

38 引用 (Scopus)

抄録

Transmembrane AMPA receptor regulatory proteins (TARPs), including stargazin/γ-2, are associated with AMPA receptors and participate in their surface delivery and anchoring at the postsynaptic membrane. TARPs may also act as a positive modulator of the AMPA receptor ion channel function; however, little is known about other TARP members except for stargazin/γ-2. We examined the synaptic localization of stargazin/γ-2 and γ-8 by immunoelectron microscopy and biochemical analysis. The analysis of sodium dodecyl sulfate-digested freeze-fracture replica labeling revealed that stargazin/γ-2 was concentrated in the postsynaptic area, whereas γ-8 was distributed both in synaptic and extra-synaptic plasma membranes of the hippocampal neuron. When a synaptic plasma membrane-enriched brain fraction was treated with Triton X-100 and separated by sucrose density gradient ultracentrifugation, a large proportion of NMDA receptor and stargazin/γ-2 was accumulated in raft-enriched fractions, whereas AMPA receptor and γ-8 were distributed in both the raft-enriched fractions and other Triton-insoluble fractions. Phosphorylation of stargazin/γ-2 and γ-8 was regulated by different sets of kinases and phosphatases in cultured cortical neurons. These results suggested that stargazin/γ-2 and γ-8 have distinct roles in postsynaptic membranes under the regulation of different intracellular signaling pathways.

元の言語英語
ページ(範囲)45-53
ページ数9
ジャーナルNeuroscience Research
55
発行部数1
DOI
出版物ステータス出版済み - 5 1 2006

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AMPA Receptors
Cell Membrane
Neurons
Synaptic Membranes
Proteins
Membranes
Immunoelectron Microscopy
Ultracentrifugation
Octoxynol
N-Methyl-D-Aspartate Receptors
Ion Channels
Phosphoric Monoester Hydrolases
Sodium Dodecyl Sulfate
Sucrose
Phosphotransferases
Phosphorylation
Brain

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)

これを引用

Differential localization and regulation of stargazin-like protein, γ-8 and stargazin in the plasma membrane of hippocampal and cortical neurons. / Inamura, Mihoko; Itakura, Makoto; Okamoto, Hirotsugu; Hoka, Sumio; Mizoguchi, Akira; Fukazawa, Yugo; Shigemoto, Ryuichi; Yamamori, Saori; Takahashi, Masami.

:: Neuroscience Research, 巻 55, 番号 1, 01.05.2006, p. 45-53.

研究成果: ジャーナルへの寄稿記事

Inamura, M, Itakura, M, Okamoto, H, Hoka, S, Mizoguchi, A, Fukazawa, Y, Shigemoto, R, Yamamori, S & Takahashi, M 2006, 'Differential localization and regulation of stargazin-like protein, γ-8 and stargazin in the plasma membrane of hippocampal and cortical neurons', Neuroscience Research, 巻. 55, 番号 1, pp. 45-53. https://doi.org/10.1016/j.neures.2006.01.004
Inamura, Mihoko ; Itakura, Makoto ; Okamoto, Hirotsugu ; Hoka, Sumio ; Mizoguchi, Akira ; Fukazawa, Yugo ; Shigemoto, Ryuichi ; Yamamori, Saori ; Takahashi, Masami. / Differential localization and regulation of stargazin-like protein, γ-8 and stargazin in the plasma membrane of hippocampal and cortical neurons. :: Neuroscience Research. 2006 ; 巻 55, 番号 1. pp. 45-53.
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AU - Mizoguchi, Akira

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