Diffusion studies on permeable nitroxyl spin probe through lipid bilayer membrane

A. Milton Franklin Benial, V. Meenakumari, Kazuhiro Ichikawa, Ken Ichi Yamada, Fuminori Hyodo, A. Jawahar, Hideo Utsumi

研究成果: 著書/レポートタイプへの貢献会議での発言

抄録

Electron spin resonance (ESR) studies were carried out for 2mM 14N labeled deutrated permeable 3- methoxycarbonyl-2,2,5,5-tetramethyl-pyrrolidine-1-oxyl (MC-PROXYL) in pure water, 1 mM, 2 mM, 3 mM and 4 mM concentration of MC-PROXYL in 300 mM concentration of liposomal solution by using a L-band ESR spectrometer. The ESR parameters such as linewidth, hyperfine coupling constant, g-factor, partition parameter and permeability were reported. The partition parameter and permeability values indicate the maximum spin distribution in the lipid phase at 2 mM concentration. This study illustrates that ESR can be used to differentiate between the intra and extra-membrane water by loading the liposome vesicles with a lipid-permeable nitroxyl spin probe. From the ESR results, the radical concentration was optimized as 2 mM in liposomal solution for ESR phantom studies and experiments.

元の言語英語
ホスト出版物のタイトルSolid State Physics - Proceedings of the 58th DAE Solid State Physics Symposium 2013
出版者American Institute of Physics Inc.
ページ222-224
ページ数3
ISBN(印刷物)9780735412255
DOI
出版物ステータス出版済み - 1 1 2014
イベント58th DAE Solid State Physics Symposium 2013 - Patiala, Punjab, インド
継続期間: 12 17 201312 21 2013

出版物シリーズ

名前AIP Conference Proceedings
1591
ISSN(印刷物)0094-243X
ISSN(電子版)1551-7616

その他

その他58th DAE Solid State Physics Symposium 2013
インド
Patiala, Punjab
期間12/17/1312/21/13

Fingerprint

lipids
electron paramagnetic resonance
membranes
probes
partitions
permeability
ultrahigh frequencies
water
spectrometers

All Science Journal Classification (ASJC) codes

  • Physics and Astronomy(all)

これを引用

Benial, A. M. F., Meenakumari, V., Ichikawa, K., Yamada, K. I., Hyodo, F., Jawahar, A., & Utsumi, H. (2014). Diffusion studies on permeable nitroxyl spin probe through lipid bilayer membrane. : Solid State Physics - Proceedings of the 58th DAE Solid State Physics Symposium 2013 (pp. 222-224). (AIP Conference Proceedings; 巻数 1591). American Institute of Physics Inc.. https://doi.org/10.1063/1.4872551

Diffusion studies on permeable nitroxyl spin probe through lipid bilayer membrane. / Benial, A. Milton Franklin; Meenakumari, V.; Ichikawa, Kazuhiro; Yamada, Ken Ichi; Hyodo, Fuminori; Jawahar, A.; Utsumi, Hideo.

Solid State Physics - Proceedings of the 58th DAE Solid State Physics Symposium 2013. American Institute of Physics Inc., 2014. p. 222-224 (AIP Conference Proceedings; 巻 1591).

研究成果: 著書/レポートタイプへの貢献会議での発言

Benial, AMF, Meenakumari, V, Ichikawa, K, Yamada, KI, Hyodo, F, Jawahar, A & Utsumi, H 2014, Diffusion studies on permeable nitroxyl spin probe through lipid bilayer membrane. : Solid State Physics - Proceedings of the 58th DAE Solid State Physics Symposium 2013. AIP Conference Proceedings, 巻. 1591, American Institute of Physics Inc., pp. 222-224, 58th DAE Solid State Physics Symposium 2013, Patiala, Punjab, インド, 12/17/13. https://doi.org/10.1063/1.4872551
Benial AMF, Meenakumari V, Ichikawa K, Yamada KI, Hyodo F, Jawahar A その他. Diffusion studies on permeable nitroxyl spin probe through lipid bilayer membrane. : Solid State Physics - Proceedings of the 58th DAE Solid State Physics Symposium 2013. American Institute of Physics Inc. 2014. p. 222-224. (AIP Conference Proceedings). https://doi.org/10.1063/1.4872551
Benial, A. Milton Franklin ; Meenakumari, V. ; Ichikawa, Kazuhiro ; Yamada, Ken Ichi ; Hyodo, Fuminori ; Jawahar, A. ; Utsumi, Hideo. / Diffusion studies on permeable nitroxyl spin probe through lipid bilayer membrane. Solid State Physics - Proceedings of the 58th DAE Solid State Physics Symposium 2013. American Institute of Physics Inc., 2014. pp. 222-224 (AIP Conference Proceedings).
@inproceedings{62b33a041c924e28a48327c669f11df8,
title = "Diffusion studies on permeable nitroxyl spin probe through lipid bilayer membrane",
abstract = "Electron spin resonance (ESR) studies were carried out for 2mM 14N labeled deutrated permeable 3- methoxycarbonyl-2,2,5,5-tetramethyl-pyrrolidine-1-oxyl (MC-PROXYL) in pure water, 1 mM, 2 mM, 3 mM and 4 mM concentration of MC-PROXYL in 300 mM concentration of liposomal solution by using a L-band ESR spectrometer. The ESR parameters such as linewidth, hyperfine coupling constant, g-factor, partition parameter and permeability were reported. The partition parameter and permeability values indicate the maximum spin distribution in the lipid phase at 2 mM concentration. This study illustrates that ESR can be used to differentiate between the intra and extra-membrane water by loading the liposome vesicles with a lipid-permeable nitroxyl spin probe. From the ESR results, the radical concentration was optimized as 2 mM in liposomal solution for ESR phantom studies and experiments.",
author = "Benial, {A. Milton Franklin} and V. Meenakumari and Kazuhiro Ichikawa and Yamada, {Ken Ichi} and Fuminori Hyodo and A. Jawahar and Hideo Utsumi",
year = "2014",
month = "1",
day = "1",
doi = "10.1063/1.4872551",
language = "English",
isbn = "9780735412255",
series = "AIP Conference Proceedings",
publisher = "American Institute of Physics Inc.",
pages = "222--224",
booktitle = "Solid State Physics - Proceedings of the 58th DAE Solid State Physics Symposium 2013",

}

TY - GEN

T1 - Diffusion studies on permeable nitroxyl spin probe through lipid bilayer membrane

AU - Benial, A. Milton Franklin

AU - Meenakumari, V.

AU - Ichikawa, Kazuhiro

AU - Yamada, Ken Ichi

AU - Hyodo, Fuminori

AU - Jawahar, A.

AU - Utsumi, Hideo

PY - 2014/1/1

Y1 - 2014/1/1

N2 - Electron spin resonance (ESR) studies were carried out for 2mM 14N labeled deutrated permeable 3- methoxycarbonyl-2,2,5,5-tetramethyl-pyrrolidine-1-oxyl (MC-PROXYL) in pure water, 1 mM, 2 mM, 3 mM and 4 mM concentration of MC-PROXYL in 300 mM concentration of liposomal solution by using a L-band ESR spectrometer. The ESR parameters such as linewidth, hyperfine coupling constant, g-factor, partition parameter and permeability were reported. The partition parameter and permeability values indicate the maximum spin distribution in the lipid phase at 2 mM concentration. This study illustrates that ESR can be used to differentiate between the intra and extra-membrane water by loading the liposome vesicles with a lipid-permeable nitroxyl spin probe. From the ESR results, the radical concentration was optimized as 2 mM in liposomal solution for ESR phantom studies and experiments.

AB - Electron spin resonance (ESR) studies were carried out for 2mM 14N labeled deutrated permeable 3- methoxycarbonyl-2,2,5,5-tetramethyl-pyrrolidine-1-oxyl (MC-PROXYL) in pure water, 1 mM, 2 mM, 3 mM and 4 mM concentration of MC-PROXYL in 300 mM concentration of liposomal solution by using a L-band ESR spectrometer. The ESR parameters such as linewidth, hyperfine coupling constant, g-factor, partition parameter and permeability were reported. The partition parameter and permeability values indicate the maximum spin distribution in the lipid phase at 2 mM concentration. This study illustrates that ESR can be used to differentiate between the intra and extra-membrane water by loading the liposome vesicles with a lipid-permeable nitroxyl spin probe. From the ESR results, the radical concentration was optimized as 2 mM in liposomal solution for ESR phantom studies and experiments.

UR - http://www.scopus.com/inward/record.url?scp=84903122134&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84903122134&partnerID=8YFLogxK

U2 - 10.1063/1.4872551

DO - 10.1063/1.4872551

M3 - Conference contribution

AN - SCOPUS:84903122134

SN - 9780735412255

T3 - AIP Conference Proceedings

SP - 222

EP - 224

BT - Solid State Physics - Proceedings of the 58th DAE Solid State Physics Symposium 2013

PB - American Institute of Physics Inc.

ER -