Diffusion studies on permeable nitroxyl spin probe through lipid bilayer membrane

A. Milton Franklin Benial, V. Meenakumari, Kazuhiro Ichikawa, Ken Ichi Yamada, Fuminori Hyodo, A. Jawahar, Hideo Utsumi

研究成果: Chapter in Book/Report/Conference proceedingConference contribution

抄録

Electron spin resonance (ESR) studies were carried out for 2mM 14N labeled deutrated permeable 3- methoxycarbonyl-2,2,5,5-tetramethyl-pyrrolidine-1-oxyl (MC-PROXYL) in pure water, 1 mM, 2 mM, 3 mM and 4 mM concentration of MC-PROXYL in 300 mM concentration of liposomal solution by using a L-band ESR spectrometer. The ESR parameters such as linewidth, hyperfine coupling constant, g-factor, partition parameter and permeability were reported. The partition parameter and permeability values indicate the maximum spin distribution in the lipid phase at 2 mM concentration. This study illustrates that ESR can be used to differentiate between the intra and extra-membrane water by loading the liposome vesicles with a lipid-permeable nitroxyl spin probe. From the ESR results, the radical concentration was optimized as 2 mM in liposomal solution for ESR phantom studies and experiments.

本文言語英語
ホスト出版物のタイトルSolid State Physics - Proceedings of the 58th DAE Solid State Physics Symposium 2013
出版社American Institute of Physics Inc.
ページ222-224
ページ数3
ISBN(印刷版)9780735412255
DOI
出版ステータス出版済み - 1 1 2014
イベント58th DAE Solid State Physics Symposium 2013 - Patiala, Punjab, インド
継続期間: 12 17 201312 21 2013

出版物シリーズ

名前AIP Conference Proceedings
1591
ISSN(印刷版)0094-243X
ISSN(電子版)1551-7616

その他

その他58th DAE Solid State Physics Symposium 2013
国/地域インド
CityPatiala, Punjab
Period12/17/1312/21/13

All Science Journal Classification (ASJC) codes

  • 物理学および天文学(全般)

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