Diffusion studies on permeable nitroxyl spin probes through bilayer lipid membranes

A low frequency ESR study

V. Meenakumari, Hideo Utsumi, Kazuhiro Ichikawa, Ken-Ichi Yamada, Fuminori Hyodo, A. Jawahar, A. Milton Franklin Benial

研究成果: 著書/レポートタイプへの貢献会議での発言

抄録

Electron spin resonance (ESR) studies were carried out for permeable 2mM 14 N-labeled deutrated 3 Methoxy carbonyl-2,2,5,5-tetramethyl-pyrrolidine-1-oxyl (MC-PROXYL) in pure water and 1mM, 2mM, 3mM, 4mM concentration of 14N-labeled deutrated MC-PROXYL in 400mM concentration of liposomal solution by using a 300 MHz ESR spectrometer. The ESR parameters such as linewidth, hyperfine coupling constant, g-factor, partition parameter and permeability were reported for these samples. The line broadening was observed for the nitroxyl spin probe in the liposomal solution. The line broadening indicates that the high viscous nature of the liposomal solution. The partition parameter and permeability values indicate the maximum diffusion of nitroxyl spin probes in the bilayer lipid membranes at 2 mM concentration of nitroxyl radical. This study illustrates that ESR can be used to differentiate between the intra and extra- membrane water by loading the liposome vesicles with a lipid-permeable nitroxyl spin probe. From the ESR results, the spin probe concentration was optimized as 2mM in liposomal solution for ESR phantom studies/imaging, invivo and invitro experiments.

元の言語英語
ホスト出版物のタイトルSolid State Physics
ホスト出版物のサブタイトルProceedings of the 59th DAE Solid State Physics Symposium 2014
編集者R. Chitra, N. K. Sahoo, Dibyendu Bhattacharyya
出版者American Institute of Physics Inc.
ISBN(電子版)9780735413108
DOI
出版物ステータス出版済み - 6 24 2015
イベント59th DAE Solid State Physics Symposium, SSPS 2014 - Vellore, Tamilnadu, インド
継続期間: 12 16 201412 20 2014

出版物シリーズ

名前AIP Conference Proceedings
1665
ISSN(印刷物)0094-243X
ISSN(電子版)1551-7616

会議

会議59th DAE Solid State Physics Symposium, SSPS 2014
インド
Vellore, Tamilnadu
期間12/16/1412/20/14

Fingerprint

lipid bilayers
electron paramagnetic resonance spectroscopy
electron spin resonance
probes (equipment)
lipids
electron paramagnetic resonance
lipid
probe
membrane
membranes
low frequencies
probes
pyrrolidines
partitions
permeability
vesicle
spectrometers
water
spectrometer
image analysis

All Science Journal Classification (ASJC) codes

  • Ecology, Evolution, Behavior and Systematics
  • Ecology
  • Plant Science
  • Physics and Astronomy(all)
  • Nature and Landscape Conservation

これを引用

Meenakumari, V., Utsumi, H., Ichikawa, K., Yamada, K-I., Hyodo, F., Jawahar, A., & Benial, A. M. F. (2015). Diffusion studies on permeable nitroxyl spin probes through bilayer lipid membranes: A low frequency ESR study. : R. Chitra, N. K. Sahoo, & D. Bhattacharyya (版), Solid State Physics: Proceedings of the 59th DAE Solid State Physics Symposium 2014 [040028] (AIP Conference Proceedings; 巻数 1665). American Institute of Physics Inc.. https://doi.org/10.1063/1.4917641

Diffusion studies on permeable nitroxyl spin probes through bilayer lipid membranes : A low frequency ESR study. / Meenakumari, V.; Utsumi, Hideo; Ichikawa, Kazuhiro; Yamada, Ken-Ichi; Hyodo, Fuminori; Jawahar, A.; Benial, A. Milton Franklin.

Solid State Physics: Proceedings of the 59th DAE Solid State Physics Symposium 2014. 版 / R. Chitra; N. K. Sahoo; Dibyendu Bhattacharyya. American Institute of Physics Inc., 2015. 040028 (AIP Conference Proceedings; 巻 1665).

研究成果: 著書/レポートタイプへの貢献会議での発言

Meenakumari, V, Utsumi, H, Ichikawa, K, Yamada, K-I, Hyodo, F, Jawahar, A & Benial, AMF 2015, Diffusion studies on permeable nitroxyl spin probes through bilayer lipid membranes: A low frequency ESR study. : R Chitra, NK Sahoo & D Bhattacharyya (版), Solid State Physics: Proceedings of the 59th DAE Solid State Physics Symposium 2014., 040028, AIP Conference Proceedings, 巻. 1665, American Institute of Physics Inc., 59th DAE Solid State Physics Symposium, SSPS 2014, Vellore, Tamilnadu, インド, 12/16/14. https://doi.org/10.1063/1.4917641
Meenakumari V, Utsumi H, Ichikawa K, Yamada K-I, Hyodo F, Jawahar A その他. Diffusion studies on permeable nitroxyl spin probes through bilayer lipid membranes: A low frequency ESR study. : Chitra R, Sahoo NK, Bhattacharyya D, 編集者, Solid State Physics: Proceedings of the 59th DAE Solid State Physics Symposium 2014. American Institute of Physics Inc. 2015. 040028. (AIP Conference Proceedings). https://doi.org/10.1063/1.4917641
Meenakumari, V. ; Utsumi, Hideo ; Ichikawa, Kazuhiro ; Yamada, Ken-Ichi ; Hyodo, Fuminori ; Jawahar, A. ; Benial, A. Milton Franklin. / Diffusion studies on permeable nitroxyl spin probes through bilayer lipid membranes : A low frequency ESR study. Solid State Physics: Proceedings of the 59th DAE Solid State Physics Symposium 2014. 編集者 / R. Chitra ; N. K. Sahoo ; Dibyendu Bhattacharyya. American Institute of Physics Inc., 2015. (AIP Conference Proceedings).
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