Direct detection of gradient-eluted non-labeled amino acids using micro-HPLC with ultraviolet thermal lensing

H. Katae, S. Hirashima, A. Harata

    研究成果: Contribution to journalArticle査読

    4 被引用数 (Scopus)

    抄録

    Thermal lensing (TL) permits ultrasensitive measurements of optical absorption of analytes in very small volumes. Separation-detection conditions of non-labeled amino acids with micro-HPLC/UV absorption detector are optimized, and direct determination of non-labeled amino acids by micro-HPLC/UV-excitation TL detection in a gradient elution is successfully demonstrated. Non-labeled amol-level amino acids is detectable with the TL detection system, which has thousand times better sensitivity than a conventional UV detector.

    本文言語英語
    論文番号012122
    ジャーナルJournal of Physics: Conference Series
    214
    DOI
    出版ステータス出版済み - 2010

    All Science Journal Classification (ASJC) codes

    • 物理学および天文学(全般)

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