Direct Evidence of Abortive Lytic Infection-Mediated Establishment of Epstein-Barr Virus Latency During B-Cell Infection

Tomoki Inagaki, Yoshitaka Sato, Jumpei Ito, Mitsuaki Takaki, Yusuke Okuno, Masahiro Yaguchi, H. M.Abdullah Al Masud, Takahiro Watanabe, Kei Sato, Shingo Iwami, Takayuki Murata, Hiroshi Kimura

研究成果: Contribution to journalArticle査読

2 被引用数 (Scopus)

抄録

Viral infection induces dynamic changes in transcriptional profiles. Virus-induced and antiviral responses are intertwined during the infection. Epstein-Barr virus (EBV) is a human gammaherpesvirus that provides a model of herpesvirus latency. To measure the transcriptome changes during the establishment of EBV latency, we infected EBV-negative Akata cells with EBV-EGFP and performed transcriptome sequencing (RNA-seq) at 0, 2, 4, 7, 10, and 14 days after infection. We found transient downregulation of mitotic division-related genes, reflecting reprogramming of cell growth by EBV, and a burst of viral lytic gene expression in the early phase of infection. Experimental and mathematical investigations demonstrate that infectious virions were not produced in the pre-latent phase, suggesting the presence of an abortive lytic infection. Fate mapping using recombinant EBV provided direct evidence that the abortive lytic infection in the pre-latent phase converges to latent infection during EBV infection of B-cells, shedding light on novel roles of viral lytic gene(s) in establishing latency. Furthermore, we find that the BZLF1 protein, which is a key regulator of reactivation, was dispensable for abortive lytic infection in the pre-latent phase, suggesting the divergent regulation of viral gene expressions from a productive lytic infection.

本文言語英語
論文番号575255
ジャーナルFrontiers in Microbiology
11
DOI
出版ステータス出版済み - 1 21 2021

All Science Journal Classification (ASJC) codes

  • 微生物学
  • 微生物学(医療)

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