Direct induction of ramified microglia-like cells from human monocytes: Dynamic microglial dysfunction in Nasu-Hakola disease

Masahiro Ohgidani, Takahiro A. Kato, Daiki Setoyama, Noriaki Sagata, Ryota Hashimoto, Kazue Shigenobu, Tetsuhiko Yoshida, Kohei Hayakawa, Norihiro Shimokawa, Daisuke Miura, Hideo Utsumi, Shigenobu Kanba

研究成果: Contribution to journalArticle査読

58 被引用数 (Scopus)

抄録

Microglia have been implicated in various neurological and psychiatric disorders in rodent and human postmortem studies. However, the dynamic actions of microglia in the living human brain have not been clarified due to a lack of studies dealing with in situ microglia. Herein, we present a novel technique for developing induced microglia-like (iMG) cells from human peripheral blood cells. An optimized cocktail of cytokines, GM-CSF and IL-34, converted human monocytes into iMG cells within 14 days. The iMG cells have microglial characterizations; expressing markers, forming a ramified morphology, and phagocytic activity with various cytokine releases. To confirm clinical utilities, we developed iMG cells from a patient of Nasu-Hakola disease (NHD), which is suggested to be directly caused by microglial dysfunction, and observed that these cells from NHD express delayed but stronger inflammatory responses compared with those from the healthy control. Altogether, the iMG-technique promises to elucidate unresolved aspects of human microglia in various brain disorders.

本文言語英語
論文番号4957
ジャーナルScientific reports
4
DOI
出版ステータス出版済み - 5 14 2014

All Science Journal Classification (ASJC) codes

  • General

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