Direct interaction between amphotericin B and ergosterol in lipid bilayers as revealed by 2H NMR spectroscopy

Nobuaki Matsumori, Kazuaki Tahara, Hiroko Yamamoto, Atsushi Morooka, Mototsugu Doi, Tohru Oishi, Michio Murata

研究成果: ジャーナルへの寄稿記事

50 引用 (Scopus)

抄録

Although amphotericin B (AmB) is thought to exert its antifungal activity by forming transmembrane ion-permeable self-assemblies together with ergosterol, no previous study has directly proven AmB-ergosterol interaction. To establish the interaction, we measured 2H NMR using deuterium-labeled sterols and AmB. The 2H NMR spectra of deuterated ergosterol in palmitoyloleoylphosphatidylcholine (POPC) bilayers showed that fast axial diffusion of erogosterol was almost completely inhibited by the coexistence of AmB. Conversely, cholesterol mobility in POPC membrane was essentially unchanged with or without AmB. These results unequivocally demonstrate that ergosterol has significant interaction with AmB in POPC bilayers. In addition, we examined the mobility of AmB using deuterium-labeled AmB, and found that, although AmB is almost immobilized in sterol-free and cholesterol-containing POPC membranes, a certain ratio of AmB molecules acquires mobility in the presence of ergosterol. The similar mobility of AmB and ergosterol in POPC bilayers confirmed the idea of the direct intermolecular interaction between ergosterol and AmB.

元の言語英語
ページ(範囲)11855-11860
ページ数6
ジャーナルJournal of the American Chemical Society
131
発行部数33
DOI
出版物ステータス出版済み - 8 26 2009
外部発表Yes

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Ergosterol
Lipid bilayers
Cholesterol
Lipid Bilayers
Amphotericin B
Deuterium
Nuclear magnetic resonance spectroscopy
Magnetic Resonance Spectroscopy
Nuclear magnetic resonance
Membranes
Self assembly
Molecules
Ions
Sterols

All Science Journal Classification (ASJC) codes

  • Catalysis
  • Chemistry(all)
  • Biochemistry
  • Colloid and Surface Chemistry

これを引用

Direct interaction between amphotericin B and ergosterol in lipid bilayers as revealed by 2H NMR spectroscopy. / Matsumori, Nobuaki; Tahara, Kazuaki; Yamamoto, Hiroko; Morooka, Atsushi; Doi, Mototsugu; Oishi, Tohru; Murata, Michio.

:: Journal of the American Chemical Society, 巻 131, 番号 33, 26.08.2009, p. 11855-11860.

研究成果: ジャーナルへの寄稿記事

Matsumori, Nobuaki ; Tahara, Kazuaki ; Yamamoto, Hiroko ; Morooka, Atsushi ; Doi, Mototsugu ; Oishi, Tohru ; Murata, Michio. / Direct interaction between amphotericin B and ergosterol in lipid bilayers as revealed by 2H NMR spectroscopy. :: Journal of the American Chemical Society. 2009 ; 巻 131, 番号 33. pp. 11855-11860.
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abstract = "Although amphotericin B (AmB) is thought to exert its antifungal activity by forming transmembrane ion-permeable self-assemblies together with ergosterol, no previous study has directly proven AmB-ergosterol interaction. To establish the interaction, we measured 2H NMR using deuterium-labeled sterols and AmB. The 2H NMR spectra of deuterated ergosterol in palmitoyloleoylphosphatidylcholine (POPC) bilayers showed that fast axial diffusion of erogosterol was almost completely inhibited by the coexistence of AmB. Conversely, cholesterol mobility in POPC membrane was essentially unchanged with or without AmB. These results unequivocally demonstrate that ergosterol has significant interaction with AmB in POPC bilayers. In addition, we examined the mobility of AmB using deuterium-labeled AmB, and found that, although AmB is almost immobilized in sterol-free and cholesterol-containing POPC membranes, a certain ratio of AmB molecules acquires mobility in the presence of ergosterol. The similar mobility of AmB and ergosterol in POPC bilayers confirmed the idea of the direct intermolecular interaction between ergosterol and AmB.",
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AU - Yamamoto, Hiroko

AU - Morooka, Atsushi

AU - Doi, Mototsugu

AU - Oishi, Tohru

AU - Murata, Michio

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