Direct isopropanol production from cellobiose by engineered Escherichia coli using a synthetic pathway and a cell surface display system

Yuki Soma, Kentaro Inokuma, Tsutomu Tanaka, Chiaki Ogino, Akihiko Kondo, Masahiro Okamoto, Taizo Hanai

研究成果: ジャーナルへの寄稿記事

38 引用 (Scopus)

抄録

Efficient bio-production from lignocellulosic biomass is required for the purpose of developing an inexpensive, practical bio-refinery process. As one approach to address this problem, we genetically engineered . Escherichia coli to produce isopropanol directly from cellobiose via the cellobiose degradation by Beta-Glucosidase (BGL) on the cell surface. First, we investigated the cellobiose consumption of two . E. coli strains with the BGL protein from . Thermobifida fusca YX (Tfu0937) fused to the anchor protein Blc (Tfu0937/Blc) using different fusion sites. Next, we introduced the synthetic pathway for isopropanol production into those strains and compared their isopropanol production in the presence of glucose. Based on the results of these assays, TA212/pTA411, which was introduced Tfu-Blc fused protein expression system and the synthetic pathway for isopropanol production, was selected for the directly isopropanol production from cellobiose. TA212/pTA411 produced 69.0. ±. 11.6. mM isopropanol at 21. h of fermentation, whereas TA212/pTA147, which did not introduced the BGL/anchor fused protein but was introduced the synthetic pathway for isopropanol production, showed no cellobiose consumption and no isopropanol production during fermentation. To our knowledge, this is the first report of the production of a bio-product from cellobiose using . E. coli.

元の言語英語
ページ(範囲)80-85
ページ数6
ジャーナルJournal of Bioscience and Bioengineering
114
発行部数1
DOI
出版物ステータス出版済み - 7 1 2012

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Cellobiose
2-Propanol
Escherichia coli
Display devices
beta-Glucosidase
Proteins
Anchors
Fermentation
Biomass
Glucose
Assays
Fusion reactions
Degradation

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

これを引用

Direct isopropanol production from cellobiose by engineered Escherichia coli using a synthetic pathway and a cell surface display system. / Soma, Yuki; Inokuma, Kentaro; Tanaka, Tsutomu; Ogino, Chiaki; Kondo, Akihiko; Okamoto, Masahiro; Hanai, Taizo.

:: Journal of Bioscience and Bioengineering, 巻 114, 番号 1, 01.07.2012, p. 80-85.

研究成果: ジャーナルへの寄稿記事

Soma, Yuki ; Inokuma, Kentaro ; Tanaka, Tsutomu ; Ogino, Chiaki ; Kondo, Akihiko ; Okamoto, Masahiro ; Hanai, Taizo. / Direct isopropanol production from cellobiose by engineered Escherichia coli using a synthetic pathway and a cell surface display system. :: Journal of Bioscience and Bioengineering. 2012 ; 巻 114, 番号 1. pp. 80-85.
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