Direct light-up of cAMP derivatives in living cells by click reactions

Kenichiro Ito, Hongshan Liu, Makoto Komiyama, Tetsuya Hayashi, Yan Xu

研究成果: Contribution to journalArticle査読

4 被引用数 (Scopus)

抄録

8-Azidoadenosine 3′,5′-cyclic monophosphate (8-azido cAMP) was directly detected in living cells, by applying Cu-free azide-alkyne cycloaddition to probe cAMP derivatives by fluorescence light-up. Fluorescence emission was generated by two non-fluorescent molecules, 8-azido cAMP as a model target and difluorinated cyclooctyne (DIFO) reagent as a probe. The azide-alkyne cycloaddition reaction between 8-azido cAMP and DIFO induces fluorescence in 8-azido cAMP. The fluorescence emission serves as a way to probe 8-azido cAMP in cells.

本文言語英語
ページ(範囲)12909-12915
ページ数7
ジャーナルMolecules
18
10
DOI
出版ステータス出版済み - 10 2013
外部発表はい

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Chemistry (miscellaneous)
  • Molecular Medicine
  • Pharmaceutical Science
  • Drug Discovery
  • Physical and Theoretical Chemistry
  • Organic Chemistry

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