Direct lineage reprogramming of mouse fibroblasts to acquire the identity of fetal intestine-derived progenitor cells

研究成果: Chapter in Book/Report/Conference proceedingChapter

抄録

Intestinal organoids are useful models for studying the characteristics of intestinal diseases and their treatment. However, a major limiting factor in their usability is the need for donor tissue fragments or pluripotent stem cells to generate the organoids. Here, we describe an approach to generate intestinal organoids from fibroblasts, a new source. We used direct reprogramming technology to generate cells with the properties of fetal intestine-derived progenitor cells (FIPCs) from mouse embryonic fibroblasts (MEFs). These induced FIPCs (iFIPCs) can give rise to cells resembling intestinal stem cells (ISCs), henceforth referred to as induced ISCs (iISCs). These iFIPCs and iISCs form spherical and budding organoids, respectively, similar to FIPCs and ISCs. These induced intestinal organoids could be used for studies on intestinal diseases and regenerative therapy.

本文言語英語
ホスト出版物のタイトルMethods in Molecular Biology
出版社Humana Press Inc.
ページ231-236
ページ数6
DOI
出版ステータス出版済み - 2020

出版物シリーズ

名前Methods in Molecular Biology
2171
ISSN(印刷版)1064-3745
ISSN(電子版)1940-6029

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics

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