TY - CHAP
T1 - Direct lineage reprogramming of mouse fibroblasts to acquire the identity of fetal intestine-derived progenitor cells
AU - Miura, Shizuka
AU - Suzuki, Atsushi
N1 - Funding Information:
We thank Drs. Toshio Kitamura and Masafumi Onodera for sharing the Plat-E cells and pGCDNsam plasmid, respectively. This work was supported in part by the JSPS KAKENHI (Grant Numbers: 23112002, 25713014, JP16H01850, JP18H06069, FDG6J02459, JP18H05102, JP19H01177, and JP19H05267), the Core Research for Evolutional Science and Technology (CREST) Program of the Japan Agency for Medical Research and Development (AMED), the Practical Research Project for Rare/ Intractable Diseases of AMED, the Research Center Network for Realization of Regenerative Medicine of AMED, and the Takeda Science Foundation.
Publisher Copyright:
© Springer Science+Business Media, LLC, part of Springer Nature 2020.
Copyright:
Copyright 2020 Elsevier B.V., All rights reserved.
PY - 2020
Y1 - 2020
N2 - Intestinal organoids are useful models for studying the characteristics of intestinal diseases and their treatment. However, a major limiting factor in their usability is the need for donor tissue fragments or pluripotent stem cells to generate the organoids. Here, we describe an approach to generate intestinal organoids from fibroblasts, a new source. We used direct reprogramming technology to generate cells with the properties of fetal intestine-derived progenitor cells (FIPCs) from mouse embryonic fibroblasts (MEFs). These induced FIPCs (iFIPCs) can give rise to cells resembling intestinal stem cells (ISCs), henceforth referred to as induced ISCs (iISCs). These iFIPCs and iISCs form spherical and budding organoids, respectively, similar to FIPCs and ISCs. These induced intestinal organoids could be used for studies on intestinal diseases and regenerative therapy.
AB - Intestinal organoids are useful models for studying the characteristics of intestinal diseases and their treatment. However, a major limiting factor in their usability is the need for donor tissue fragments or pluripotent stem cells to generate the organoids. Here, we describe an approach to generate intestinal organoids from fibroblasts, a new source. We used direct reprogramming technology to generate cells with the properties of fetal intestine-derived progenitor cells (FIPCs) from mouse embryonic fibroblasts (MEFs). These induced FIPCs (iFIPCs) can give rise to cells resembling intestinal stem cells (ISCs), henceforth referred to as induced ISCs (iISCs). These iFIPCs and iISCs form spherical and budding organoids, respectively, similar to FIPCs and ISCs. These induced intestinal organoids could be used for studies on intestinal diseases and regenerative therapy.
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U2 - 10.1007/978-1-0716-0747-3_14
DO - 10.1007/978-1-0716-0747-3_14
M3 - Chapter
C2 - 32705645
AN - SCOPUS:85088538437
T3 - Methods in Molecular Biology
SP - 231
EP - 236
BT - Methods in Molecular Biology
PB - Humana Press Inc.
ER -