Direct Monitoring of γ-Glutamyl Transpeptidase Activity In Vivo Using a Hyperpolarized13C-Labeled Molecular Probe

Tatsuya Nishihara, Hikari A.I. Yoshihara, Hiroshi Nonaka, Yoichi Takakusagi, Fuminori Hyodo, Kazuhiro Ichikawa, Emine Can, Jessica A.M. Bastiaansen, Yuhei Takado, Arnaud Comment, Shinsuke Sando

    研究成果: Contribution to journalArticle査読

    22 被引用数 (Scopus)

    抄録

    The γ-glutamyl transpeptidase (GGT) enzyme plays a central role in glutathione homeostasis. Direct detection of GGT activity could provide critical information for the diagnosis of several pathologies. We propose a new molecular probe, γ-Glu-[1-13C]Gly, for monitoring GGT activity in vivo by hyperpolarized (HP)13C magnetic resonance (MR). The properties of γ-Glu-[1-13C]Gly are suitable for in vivo HP13C metabolic analysis since the chemical shift between γ-Glu-[1-13C]Gly and its metabolic product, [1-13C]Gly, is large (4.3 ppm) and the T1of both compounds is relatively long (30 s and 45 s, respectively, in H2O at 9.4 T). We also demonstrate that γ-Glu-[1-13C]Gly is highly sensitive to in vivo modulation of GGT activity induced by the inhibitor acivicin.

    本文言語英語
    ページ(範囲)10626-10629
    ページ数4
    ジャーナルAngewandte Chemie - International Edition
    55
    36
    DOI
    出版ステータス出版済み - 8 26 2016

    All Science Journal Classification (ASJC) codes

    • 触媒
    • 化学 (全般)

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