Direct stimulation of cellular immune response via TLR2 signaling triggered by contact with hybrid glyco-biointerfaces composed of chitohexaose and cellohexaose

Mayumi Hatakeyama, Fumi Nakada, Hirofumi Ichinose, Takuya Kitaoka

研究成果: ジャーナルへの寄稿記事

抄録

Recognition of carbohydrates by cell receptors activates various cell signaling processes. In this report, hybrid self-assembled monolayers (SAMs) of chitohexaose (βGlcNAc6) and cellohexaose (βGlc6) are prepared to induce a highly sensitive, glyco-density-dependent inflammatory response by HEK293 cells expressing toll-like receptor 2 (TLR2), which recognizes oligo-βGlcNAc moieties. Thiosemicarbazide-labeled βGlcNAc6 and βGlc6, as a bio-signal inducer and a spacer molecule, respectively, were immobilized on a gold substrate via spontaneous chemisorption to control the βGlcNAc6 density on the SAM surface. The βGlcNAc6 density ranged from 0 to 0.65 chains nm –2 . The inflammatory response of HEK293 cells varied as a function of the surface glyco-density, and the hybrid SAM with 0.146 chains nm –2 of βGlcNAc6 induced a stronger response than pure βGlcNAc6-SAM with 0.654 chains nm –2 . Thus, direct activation of TLR2-mediated cellular stimulation triggered by as-designed glyco-biointerfaces was possible through a mutual interaction between the fittingly βGlcNAc6 moieties assembled on hybrid glyco-SAMs and TLR2 on the surface of HEK293 cells.

元の言語英語
ページ(範囲)517-522
ページ数6
ジャーナルColloids and Surfaces B: Biointerfaces
175
DOI
出版物ステータス出版済み - 3 1 2019

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Toll-Like Receptor 2
HEK293 Cells
Self assembled monolayers
stimulation
Cellular Immunity
cells
Gold
carbohydrates
Cell signaling
Carbohydrates
spacers
chemisorption
Chemisorption
activation
gold
Chemical activation
Cells
cellohexaose
chitohexaose
Molecules

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Surfaces and Interfaces
  • Physical and Theoretical Chemistry
  • Colloid and Surface Chemistry

これを引用

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abstract = "Recognition of carbohydrates by cell receptors activates various cell signaling processes. In this report, hybrid self-assembled monolayers (SAMs) of chitohexaose (βGlcNAc6) and cellohexaose (βGlc6) are prepared to induce a highly sensitive, glyco-density-dependent inflammatory response by HEK293 cells expressing toll-like receptor 2 (TLR2), which recognizes oligo-βGlcNAc moieties. Thiosemicarbazide-labeled βGlcNAc6 and βGlc6, as a bio-signal inducer and a spacer molecule, respectively, were immobilized on a gold substrate via spontaneous chemisorption to control the βGlcNAc6 density on the SAM surface. The βGlcNAc6 density ranged from 0 to 0.65 chains nm –2 . The inflammatory response of HEK293 cells varied as a function of the surface glyco-density, and the hybrid SAM with 0.146 chains nm –2 of βGlcNAc6 induced a stronger response than pure βGlcNAc6-SAM with 0.654 chains nm –2 . Thus, direct activation of TLR2-mediated cellular stimulation triggered by as-designed glyco-biointerfaces was possible through a mutual interaction between the fittingly βGlcNAc6 moieties assembled on hybrid glyco-SAMs and TLR2 on the surface of HEK293 cells.",
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T1 - Direct stimulation of cellular immune response via TLR2 signaling triggered by contact with hybrid glyco-biointerfaces composed of chitohexaose and cellohexaose

AU - Hatakeyama, Mayumi

AU - Nakada, Fumi

AU - Ichinose, Hirofumi

AU - Kitaoka, Takuya

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AB - Recognition of carbohydrates by cell receptors activates various cell signaling processes. In this report, hybrid self-assembled monolayers (SAMs) of chitohexaose (βGlcNAc6) and cellohexaose (βGlc6) are prepared to induce a highly sensitive, glyco-density-dependent inflammatory response by HEK293 cells expressing toll-like receptor 2 (TLR2), which recognizes oligo-βGlcNAc moieties. Thiosemicarbazide-labeled βGlcNAc6 and βGlc6, as a bio-signal inducer and a spacer molecule, respectively, were immobilized on a gold substrate via spontaneous chemisorption to control the βGlcNAc6 density on the SAM surface. The βGlcNAc6 density ranged from 0 to 0.65 chains nm –2 . The inflammatory response of HEK293 cells varied as a function of the surface glyco-density, and the hybrid SAM with 0.146 chains nm –2 of βGlcNAc6 induced a stronger response than pure βGlcNAc6-SAM with 0.654 chains nm –2 . Thus, direct activation of TLR2-mediated cellular stimulation triggered by as-designed glyco-biointerfaces was possible through a mutual interaction between the fittingly βGlcNAc6 moieties assembled on hybrid glyco-SAMs and TLR2 on the surface of HEK293 cells.

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