Direction and speed of microtubule movements driven by kinesin motors arranged on catchin thick filaments

Toshiki Taba, Masaki Edamatsu, Shiori Toba, Keitaro Shibata, Yasuhiro Imafuku, Yoko Yano Toyoshima, Katsuhisa Tawada, Akira Yamada

研究成果: ジャーナルへの寄稿記事

3 引用 (Scopus)

抄録

Conventional kinesin (Kinesin-1) is a microtubule-based molecular motor that supports intracellular vesicle/organelle transport in various eukaryotic cells. To arrange kinesin motors similarly to myosin motors on thick filaments in muscles, the motor domain of rat conventional kinesin (amino acid residues 1-430) fused to the C-terminal 829 amino acid residues of catchin (KHC430Cat) was bacterially expressed and attached to catchin filaments that can attach to and arrange myosin molecules in a bipolar manner on their surface. Unlike the case of myosin where actin filaments move toward the center much faster than in the opposite direction along the catchin filaments, microtubules moved at the same speed in both directions. In addition, many microtubules moved across the filaments at the same speed with various angles between the axes of the microtubule and catchin filament. Kinesin/catchin chimera proteins with a shorter kinesin neck domain were also prepared. Those without the whole hinge 1 domain and the C-terminal part of the neck helix moved microtubules toward the center of the catchin filaments significantly, but only slightly, faster than in the opposite direction, although the movements in both directions were slower than those of the KHC430Cat construct. The results suggest that kinesin has substantial mechanical flexibility within the motor domain, possibly within the neck linker, enabling its interaction with microtubules having any orientation.

元の言語英語
ページ(範囲)816-826
ページ数11
ジャーナルCell Motility and the Cytoskeleton
65
発行部数10
DOI
出版物ステータス出版済み - 10 1 2008

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Kinesin
Microtubules
Myosins
Neck
Amino Acids
Transport Vesicles
Eukaryotic Cells
Direction compound
Actin Cytoskeleton
Organelles
Muscles

All Science Journal Classification (ASJC) codes

  • Structural Biology
  • Cell Biology

これを引用

Direction and speed of microtubule movements driven by kinesin motors arranged on catchin thick filaments. / Taba, Toshiki; Edamatsu, Masaki; Toba, Shiori; Shibata, Keitaro; Imafuku, Yasuhiro; Toyoshima, Yoko Yano; Tawada, Katsuhisa; Yamada, Akira.

:: Cell Motility and the Cytoskeleton, 巻 65, 番号 10, 01.10.2008, p. 816-826.

研究成果: ジャーナルへの寄稿記事

Taba, T, Edamatsu, M, Toba, S, Shibata, K, Imafuku, Y, Toyoshima, YY, Tawada, K & Yamada, A 2008, 'Direction and speed of microtubule movements driven by kinesin motors arranged on catchin thick filaments', Cell Motility and the Cytoskeleton, 巻. 65, 番号 10, pp. 816-826. https://doi.org/10.1002/cm.20303
Taba, Toshiki ; Edamatsu, Masaki ; Toba, Shiori ; Shibata, Keitaro ; Imafuku, Yasuhiro ; Toyoshima, Yoko Yano ; Tawada, Katsuhisa ; Yamada, Akira. / Direction and speed of microtubule movements driven by kinesin motors arranged on catchin thick filaments. :: Cell Motility and the Cytoskeleton. 2008 ; 巻 65, 番号 10. pp. 816-826.
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