Discovery of coumarin as the predominant allelochemical in Guricidia sepium

T. Takemura, T. Kamo, E. Sakuno, Syuntaro Hiradate, Y. Fujii

研究成果: Contribution to journalArticle査読

21 被引用数 (Scopus)

抄録

A crude methanol extract of Gliricidia sepium (Fabaceae) leaves inhibited the growth of lettuce (Lactuca sativa) radicles. To isolate and characterise the inhibitory compound, the extract was fractionated based on the total activity on lettuce radicle elongation. The n-hexane-soluble fraction obtained by liquid-liquid partitioning of the crude methanol extract showed strong inhibitory activity. A compound corresponding to the major peak in high performance liquid chromatography was isolated from the fraction and identified as coumarin. The EC50 of coumarin for the growth of lettuce radicles was 23.3 umol L-1. On the basis of the coumarin content (11.6 mmol kg-1 fresh weight) and the total activity of coumarin (500) in G. sepium, we concluded that the inhibitory activity of G. sepium was primarily due to coumarin.

本文言語英語
ページ(範囲)268-272
ページ数5
ジャーナルJournal of Tropical Forest Science
25
2
出版ステータス出版済み - 4 30 2013
外部発表はい

All Science Journal Classification (ASJC) codes

  • 林業

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