Discovery of highly reactive peptide tag by ELISA-type screening for specific cysteine conjugation

Nobutaka Kurashige, Hirokazu Fuchida, Shigekazu Tabata, Shohei Uchinomiya, Akio Ojida

研究成果: ジャーナルへの寄稿記事

1 引用 (Scopus)

抄録

We report the discovery of a highly reactive peptide tag for the specific cysteine conjugation of proteins. Screening of cysteine-containing peptides using ELISA-type screening yielded a 19-amino acid tag (DCPPPDDAADDAADDAADD), named DCP3 tag, which enabled the rapid and selective labeling of the tag-fused protein with a synthetic zinc complex on the surface of living cells.

元の言語英語
ジャーナルBioorganic and Medicinal Chemistry Letters
DOI
出版物ステータス受理済み/印刷中 - 4 18 2017

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Cysteine
Screening
Enzyme-Linked Immunosorbent Assay
Peptides
Labeling
Zinc
Proteins
Cells
Amino Acids

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Medicine
  • Molecular Biology
  • Pharmaceutical Science
  • Drug Discovery
  • Clinical Biochemistry
  • Organic Chemistry

これを引用

Discovery of highly reactive peptide tag by ELISA-type screening for specific cysteine conjugation. / Kurashige, Nobutaka; Fuchida, Hirokazu; Tabata, Shigekazu; Uchinomiya, Shohei; Ojida, Akio.

:: Bioorganic and Medicinal Chemistry Letters, 18.04.2017.

研究成果: ジャーナルへの寄稿記事

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