Discrimination between stable and dynamic components of protein complexes by means of quantitative proteomics

Keiji Kito, Noriko Kawaguchi, Satoshi Okada, Takashi Ito

研究成果: Contribution to journalArticle査読

13 被引用数 (Scopus)

抄録

To discriminate between stable and dynamic protein-protein interactions, we propose a strategy in which cells with and without tagged bait are differentially labeled with stable isotope and combined prior to complex purification. Mass-spectrometric analysis of the purified complexes identifies stable and dynamic components as those derived exclusively from the tagged cells and those from both cells, respectively. We successfully applied this strategy to analyze two yeast protein complexes, eIF2B-eIF2 and cyclin-Cdc28.

本文言語英語
ページ(範囲)2366-2370
ページ数5
ジャーナルProteomics
8
12
DOI
出版ステータス出版済み - 6 1 2008
外部発表はい

All Science Journal Classification (ASJC) codes

  • 生化学
  • 分子生物学

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