抄録
To discriminate between stable and dynamic protein-protein interactions, we propose a strategy in which cells with and without tagged bait are differentially labeled with stable isotope and combined prior to complex purification. Mass-spectrometric analysis of the purified complexes identifies stable and dynamic components as those derived exclusively from the tagged cells and those from both cells, respectively. We successfully applied this strategy to analyze two yeast protein complexes, eIF2B-eIF2 and cyclin-Cdc28.
| 元の言語 | 英語 |
|---|---|
| ページ（範囲） | 2366-2370 |
| ページ数 | 5 |
| ジャーナル | Proteomics |
| 巻 | 8 |
| 発行部数 | 12 |
| DOI | |
| 出版物ステータス | 出版済み - 6 1 2008 |
| 外部発表 | Yes |
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All Science Journal Classification (ASJC) codes
- Biochemistry
- Molecular Biology
これを引用
Discrimination between stable and dynamic components of protein complexes by means of quantitative proteomics. / Kito, Keiji; Kawaguchi, Noriko; Okada, Satoshi; Ito, Takashi.
：: Proteomics, 巻 8, 番号 12, 01.06.2008, p. 2366-2370.研究成果: ジャーナルへの寄稿 › 記事
}
TY - JOUR
T1 - Discrimination between stable and dynamic components of protein complexes by means of quantitative proteomics
AU - Kito, Keiji
AU - Kawaguchi, Noriko
AU - Okada, Satoshi
AU - Ito, Takashi
PY - 2008/6/1
Y1 - 2008/6/1
N2 - To discriminate between stable and dynamic protein-protein interactions, we propose a strategy in which cells with and without tagged bait are differentially labeled with stable isotope and combined prior to complex purification. Mass-spectrometric analysis of the purified complexes identifies stable and dynamic components as those derived exclusively from the tagged cells and those from both cells, respectively. We successfully applied this strategy to analyze two yeast protein complexes, eIF2B-eIF2 and cyclin-Cdc28.
AB - To discriminate between stable and dynamic protein-protein interactions, we propose a strategy in which cells with and without tagged bait are differentially labeled with stable isotope and combined prior to complex purification. Mass-spectrometric analysis of the purified complexes identifies stable and dynamic components as those derived exclusively from the tagged cells and those from both cells, respectively. We successfully applied this strategy to analyze two yeast protein complexes, eIF2B-eIF2 and cyclin-Cdc28.
UR - http://www.scopus.com/inward/record.url?scp=48249094218&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=48249094218&partnerID=8YFLogxK
U2 - 10.1002/pmic.200800182
DO - 10.1002/pmic.200800182
M3 - Article
C2 - 18563728
AN - SCOPUS:48249094218
VL - 8
SP - 2366
EP - 2370
JO - Proteomics
JF - Proteomics
SN - 1615-9853
IS - 12
ER -