Discrimination between stable and dynamic components of protein complexes by means of quantitative proteomics

Keiji Kito, Noriko Kawaguchi, Satoshi Okada, Takashi Ito

研究成果: ジャーナルへの寄稿記事

13 引用 (Scopus)

抄録

To discriminate between stable and dynamic protein-protein interactions, we propose a strategy in which cells with and without tagged bait are differentially labeled with stable isotope and combined prior to complex purification. Mass-spectrometric analysis of the purified complexes identifies stable and dynamic components as those derived exclusively from the tagged cells and those from both cells, respectively. We successfully applied this strategy to analyze two yeast protein complexes, eIF2B-eIF2 and cyclin-Cdc28.

元の言語英語
ページ(範囲)2366-2370
ページ数5
ジャーナルProteomics
8
発行部数12
DOI
出版物ステータス出版済み - 6 1 2008
外部発表Yes

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Proteomics
Cyclins
Fungal Proteins
Isotopes
Purification
Proteins

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology

これを引用

Discrimination between stable and dynamic components of protein complexes by means of quantitative proteomics. / Kito, Keiji; Kawaguchi, Noriko; Okada, Satoshi; Ito, Takashi.

:: Proteomics, 巻 8, 番号 12, 01.06.2008, p. 2366-2370.

研究成果: ジャーナルへの寄稿記事

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