TY - JOUR
T1 - Disruption of blood-retinal barrier in experimental diabetic rats
T2 - An electron microscopic study
AU - Ishibashi, Tatsuro
AU - Tanaka, Kenzo
AU - Taniguchi, Yoshiaki
PY - 1980/4
Y1 - 1980/4
N2 - Permeability pattern of retinal capillaries in streptozotocin-diabetic rats, maintained for 1-6 months, was studied with the electron microscope, using horseradish peroxidase as a tracer. A reaction product of horseradish peroxidase was confined to the capillary lumina and to a small number of vesicles on the luminal portion of the endothelial cells in control rats and diabetic rats maintained for 1 month. In diabetic rats maintained for 2-6 months, reaction product was observed in the basement membrane of endothelial cells and pericytes and extended to the extracellular spaces around the capillaries. The number of reaction product-labeled vesicles in the capillary endothelial cells was greatly increased in diabetic rats maintained for 2 months, as compared with that in control rats and diabetic rats maintained for 1 month. Reaction product was also found in the intercellular junctions of capillary endothelial cells from the luminal to the abluminal portion in diabetic rats maintained for 3-6 months. The increased permeability in retinal capillaries of diabetic rats preceded the thickening of the basement membrane, and seemed to play an important role in the development of diabetic retinopathy.
AB - Permeability pattern of retinal capillaries in streptozotocin-diabetic rats, maintained for 1-6 months, was studied with the electron microscope, using horseradish peroxidase as a tracer. A reaction product of horseradish peroxidase was confined to the capillary lumina and to a small number of vesicles on the luminal portion of the endothelial cells in control rats and diabetic rats maintained for 1 month. In diabetic rats maintained for 2-6 months, reaction product was observed in the basement membrane of endothelial cells and pericytes and extended to the extracellular spaces around the capillaries. The number of reaction product-labeled vesicles in the capillary endothelial cells was greatly increased in diabetic rats maintained for 2 months, as compared with that in control rats and diabetic rats maintained for 1 month. Reaction product was also found in the intercellular junctions of capillary endothelial cells from the luminal to the abluminal portion in diabetic rats maintained for 3-6 months. The increased permeability in retinal capillaries of diabetic rats preceded the thickening of the basement membrane, and seemed to play an important role in the development of diabetic retinopathy.
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U2 - 10.1016/0014-4835(80)90055-X
DO - 10.1016/0014-4835(80)90055-X
M3 - Article
C2 - 7449869
AN - SCOPUS:0018827048
VL - 30
SP - 401
EP - 410
JO - Experimental Eye Research
JF - Experimental Eye Research
SN - 0014-4835
IS - 4
ER -
