Disruption of mesodermal enhancers for Igf2 in the minute mutant

Karen Davies, Lucy Bowden, Paul Smith, Wendy Dean, David Hill, Hiroyasu Furuumi, Hiroyuki Sasaki, Bruce Cattanach, Wolf Reik

研究成果: Contribution to journalReview article査読

32 被引用数 (Scopus)

抄録

The radiation-induced mutation minute (Mnt) in the mouse leads to intrauterine growth retardation with paternal transmission and has been linked to the distal chromosome 7 cluster of imprinted genes. We show that the mutation is an inversion, whose breakpoint distal to H19 disrupts and thus identifies an enhancer for Igf2 expression in skeletal muscle and tongue, and separates the gene from other mesodermal and extra-embryonic enhancers. Paternal transmission of Mnt leads to drastic downregulation of Igf2 transcripts in all mesodermal tissues and the placenta. Maternal transmission leads to methylation of the H19 differentially methylated region (DMR) and silencing of H19, showing that elements 3′ of H19 can modify the maternal imprint. Methylation of the maternal DMR leads to biallelic expression of Igf2 in endodermal tissues and foetal overgrowth, demonstrating that methylation in vivo can open the chromatin boundary upstream of H19. Our work shows that most known enhancers for Igf2 are located 3′ of H19 and establishes an important genetic paradigm for the inheritance of complex regulatory mutations in imprinted gene clusters.

本文言語英語
ページ(範囲)1657-1668
ページ数12
ジャーナルDevelopment
129
7
出版ステータス出版済み - 8 23 2002
外部発表はい

All Science Journal Classification (ASJC) codes

  • 分子生物学
  • 発生生物学

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