Distinct roles of doublecortin modulating the microtubule cytoskeleton

Carolyn A. Moores, Mylène Perderiset, Caroline Kappeler, Susan Kain, Douglas Drummond, Stephen J. Perkins, Jamel Chelly, Rob Cross, Anne Houdusse, Fiona Francis

研究成果: ジャーナルへの寄稿記事

75 引用 (Scopus)

抄録

Doublecortin is a neuronal microtubule-stabilising protein, mutations of which cause mental retardation and epilepsy in humans. How doublecortin influences microtubule dynamics, and thereby brain development, is unclear. We show here by video microscopy that purified doublecortin has no effect on the growth rate of microtubules. However, it is a potent anti-catastrophe factor that stabilises microtubules by linking adjacent protofilaments and counteracting their outward bending in depolymerising microtubules. We show that doublecortin-stabilised microtubules are substrates for kinesin translocase motors and for depolymerase kinesins. In addition, doublecortin does not itself oligomerise and does not bind to tubulin heterodimers but does nucleate microtubules. In cells, doublecortin is enriched at the distal ends of neuronal processes and our data raise the possibility that the function of doublecortin in neurons is to drive assembly and stabilisation of non-centrosomal microtubules in these doublecortin-enriched distal zones. These distinct properties combine to give doublecortin a unique function in microtubule regulation, a role that cannot be compensated for by other microtubule- stabilising proteins and nucleating factors.

元の言語英語
ページ(範囲)4448-4457
ページ数10
ジャーナルEMBO Journal
25
発行部数19
DOI
出版物ステータス出版済み - 10 4 2006

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Kinesin
Cytoskeleton
Microtubules
Tubulin
Neurons
Brain
Microscopic examination
Proteins
Stabilization
Microtubule Proteins
Substrates
Video Microscopy
Intellectual Disability
Epilepsy
Mutation
Growth

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)
  • Molecular Biology
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

これを引用

Moores, C. A., Perderiset, M., Kappeler, C., Kain, S., Drummond, D., Perkins, S. J., ... Francis, F. (2006). Distinct roles of doublecortin modulating the microtubule cytoskeleton. EMBO Journal, 25(19), 4448-4457. https://doi.org/10.1038/sj.emboj.7601335

Distinct roles of doublecortin modulating the microtubule cytoskeleton. / Moores, Carolyn A.; Perderiset, Mylène; Kappeler, Caroline; Kain, Susan; Drummond, Douglas; Perkins, Stephen J.; Chelly, Jamel; Cross, Rob; Houdusse, Anne; Francis, Fiona.

:: EMBO Journal, 巻 25, 番号 19, 04.10.2006, p. 4448-4457.

研究成果: ジャーナルへの寄稿記事

Moores, CA, Perderiset, M, Kappeler, C, Kain, S, Drummond, D, Perkins, SJ, Chelly, J, Cross, R, Houdusse, A & Francis, F 2006, 'Distinct roles of doublecortin modulating the microtubule cytoskeleton', EMBO Journal, 巻. 25, 番号 19, pp. 4448-4457. https://doi.org/10.1038/sj.emboj.7601335
Moores CA, Perderiset M, Kappeler C, Kain S, Drummond D, Perkins SJ その他. Distinct roles of doublecortin modulating the microtubule cytoskeleton. EMBO Journal. 2006 10 4;25(19):4448-4457. https://doi.org/10.1038/sj.emboj.7601335
Moores, Carolyn A. ; Perderiset, Mylène ; Kappeler, Caroline ; Kain, Susan ; Drummond, Douglas ; Perkins, Stephen J. ; Chelly, Jamel ; Cross, Rob ; Houdusse, Anne ; Francis, Fiona. / Distinct roles of doublecortin modulating the microtubule cytoskeleton. :: EMBO Journal. 2006 ; 巻 25, 番号 19. pp. 4448-4457.
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