Distribution of substance P and neurokinin-1 receptors in the peri-implant epithelium around titanium dental implants in rats

Takayoshi Yamaza, Mizuho A. Kido, Bing Wang, Atsushi Danjo, Daiji Shimohira, Naohisa Murata, Masao Yoshinari, Teruo Tanaka

研究成果: ジャーナルへの寄稿学術誌査読

7 被引用数 (Scopus)

抄録

We examined the distribution of substance P and neurokinin-1 (NK1) receptors and substance-P-containing nerve fibers in the peri-implant mucosa around titanium dental implants in rats. Immunohistochemistry and immunocytochemistry revealed that substance-P-immunoreactive nerve fibers abundantly innervated the peri-implant epithelium (PIE) compared with other epithelia of the peri-implant mucosa. NK1 receptor mRNA and protein expression in the peri-implant mucosa were confirmed by reverse transcription with the polymerase chain reaction and immunoblotting. Immunoelectron microscopy revealed that NK1 receptor immunoreactivity was preferentially localized in peri-implant epithelial cells. NK1-receptor-positive products were found on the plasma membrane and in vesicles and granules in PIE cells. Neutrophils and intraepithelial nerve axons in the PIE were positive for the NK1 receptor. NK1 receptor immunoreactivity was also detected in endothelial cells, fibroblasts, and nerve fibers in the connective tissue beneath the PIE. These findings suggest that peri-implant tissue receives sensory information through regenerated nerves expressing substance P and the NK1 receptor. In the peri-implant mucosa, the substance P/NK1 receptor system may play a role in pain transmission, the endocytosis of neutrophils, the extravasation of crevicular fluid, and the migration of macrophages and neutrophils in response to neurogenic inflammation, as in healthy gingiva.

本文言語英語
ページ(範囲)407-415
ページ数9
ジャーナルCell and tissue research
335
2
DOI
出版ステータス出版済み - 2月 2009

!!!All Science Journal Classification (ASJC) codes

  • 病理学および法医学
  • 組織学
  • 細胞生物学

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