The distribution of taste buds of layer-type chickens, from day 1 (H1) to day 140 (H140) post-hatching, were investigated histologically and with a scanning electron microscope. Two types of openings were observed on the oral epithelium: large openings that were greater than 10 μm in width, and had an ellipsoid or spindle shape; and small openings that were less than 7 μm in width, and had a circular or polygon shape. It was defined that the former represent the openings of the salivary glands, and the latter are those of the taste buds. The number of taste buds remained constant from H1 to H140. Therefore, no significant change was observed between these two time points. To investigate the distribution of the taste buds in the whole oral cavity, a distribution map was created.
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