Diversity of glutelin acidic subunit polypeptides in rice cultivars collected from Northern Vietnam

Tran T.T. Hoai, Tran D. Suu, Hikaru Satoh, Toshihiro Kumamaru

研究成果: Contribution to journalArticle査読

抄録

The diversity of glutelin acidic polypeptides in rice cultivars collected from Northern Vietnam was characterized via sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) and isoelectric focusing (IEF) electrophoresis. Glutelin acidic subunits were separated into four bands by molecular mass, as α-1 (39 kDa), α-2 (38 kDa), α-3 (37.5 or 37 kDa) and α-4 (34 or 33 kDa). One hundred and eighty-five Vietnamese rice cultivars were divided into three types, based on differences in staining intensity and the molecular size of the α-3 and α-4 polypeptides derived from SDS-PAGE analysis. Wide variation was also observed in the isoelectric point (pI) staining intensity, in addition to the absence/presence of pI bands detected via IEF analysis. A total of 16 pI bands, ranging from pI 6.30 to pI 7.52, were identified in the various local rice cultivars. The maximum and minimum of IEF bands detected were 14 and 10, respectively. The genetic variability index (H) ranged from 0.280 to 0.820, which confirms that local rice cultivars from Northern Vietnam have diverse glutelin seed storage units.

本文言語英語
ページ(範囲)341-347
ページ数7
ジャーナルPlant Breeding
133
3
DOI
出版ステータス出版済み - 6 2014

All Science Journal Classification (ASJC) codes

  • 農業および作物学
  • 遺伝学
  • 植物科学

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