Diversity of glutelin acidic subunit polypeptides in rice cultivars collected from Northern Vietnam

Tran T.T. Hoai, Tran D. Suu, Hikaru Satoh, Toshihiro Kumamaru

研究成果: ジャーナルへの寄稿記事

抄録

The diversity of glutelin acidic polypeptides in rice cultivars collected from Northern Vietnam was characterized via sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) and isoelectric focusing (IEF) electrophoresis. Glutelin acidic subunits were separated into four bands by molecular mass, as α-1 (39 kDa), α-2 (38 kDa), α-3 (37.5 or 37 kDa) and α-4 (34 or 33 kDa). One hundred and eighty-five Vietnamese rice cultivars were divided into three types, based on differences in staining intensity and the molecular size of the α-3 and α-4 polypeptides derived from SDS-PAGE analysis. Wide variation was also observed in the isoelectric point (pI) staining intensity, in addition to the absence/presence of pI bands detected via IEF analysis. A total of 16 pI bands, ranging from pI 6.30 to pI 7.52, were identified in the various local rice cultivars. The maximum and minimum of IEF bands detected were 14 and 10, respectively. The genetic variability index (H) ranged from 0.280 to 0.820, which confirms that local rice cultivars from Northern Vietnam have diverse glutelin seed storage units.

元の言語英語
ページ(範囲)341-347
ページ数7
ジャーナルPlant Breeding
133
発行部数3
DOI
出版物ステータス出版済み - 1 1 2014

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glutelins
Glutens
Vietnam
polypeptides
isoelectric focusing
Isoelectric Focusing
rice
Peptides
cultivars
Sodium Dodecyl Sulfate
polyacrylamide gel electrophoresis
Polyacrylamide Gel Electrophoresis
Staining and Labeling
seed storage
Isoelectric Point
isoelectric point
electrophoresis
Electrophoresis
Seeds
molecular weight

All Science Journal Classification (ASJC) codes

  • Agronomy and Crop Science
  • Genetics
  • Plant Science

これを引用

Diversity of glutelin acidic subunit polypeptides in rice cultivars collected from Northern Vietnam. / Hoai, Tran T.T.; Suu, Tran D.; Satoh, Hikaru; Kumamaru, Toshihiro.

:: Plant Breeding, 巻 133, 番号 3, 01.01.2014, p. 341-347.

研究成果: ジャーナルへの寄稿記事

Hoai, Tran T.T. ; Suu, Tran D. ; Satoh, Hikaru ; Kumamaru, Toshihiro. / Diversity of glutelin acidic subunit polypeptides in rice cultivars collected from Northern Vietnam. :: Plant Breeding. 2014 ; 巻 133, 番号 3. pp. 341-347.
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