DNA conformation-dependent activities of human mitochondrial RNA polymerase

Atsushi Fukuoh, Kippei Ohgaki, Hinako Hatae, Isao Kuraoka, Yoshimasa Aoki, Takeshi Uchiumi, Howard T. Jacobs, Dongchon Kang

研究成果: ジャーナルへの寄稿学術誌査読

17 被引用数 (Scopus)

抄録

Mitochondrial RNA polymerase (POLRMT) is a core protein for mitochondrial DNA (mtDNA) transcription. In addition, POLRMT is assumed to be involved in replication, although its exact role is not yet clearly elucidated. We have found novel properties of human POLRMT using a reconstituted transcription system. Various lengths of RNA molecules were synthesized from templates even without a defined promoter sequence, when we used supercoiled circular double-stranded DNA as a template. This promoter-independent activity was as strong as the promoter-dependent one. Promoter-independent DNA conformation-dependent transcription required TFB2M. On supercoiled templates, the promoter-independent activity was strongly suppressed by a putatively physiological amount of TFAM, while promoter-dependent transcription was inhibited to a lesser extent. These different inhibition patterns by TFAM may be important for prevention of random RNA synthesis in vivo. Promoter-independent activity was also observed on relaxed circular single-stranded DNA, where its activity no longer required TFB2M. RNA synthesis on single-stranded DNA was weakly suppressed by a putatively physiological amount of TFAM but restored by the addition of mitochondrial single-stranded DNA binding protein. We suggest that these properties of POLRMT could explain the characteristic features of mammalian mtDNA transcription and replication.

本文言語英語
ページ(範囲)1029-1042
ページ数14
ジャーナルGenes to Cells
14
8
DOI
出版ステータス出版済み - 2009

!!!All Science Journal Classification (ASJC) codes

  • 遺伝学
  • 細胞生物学

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