DNA damage sensible engineered promoter for cellular biosensing of cytotoxicity

Ken Ichi Wada, Yu Hamaguchi, Kiyoshi Furukawa, Akiyoshi Taniguchi

研究成果: Contribution to journalArticle査読

8 被引用数 (Scopus)

抄録

We have established a cytotoxic sensor cell line by transfecting HepG2 cells with a luciferase protein plasmid derived from the heat shock protein 70B0 (HSP70B0) promoter, which is induced by cytotoxic reagents. HSP70B genes are up-regulated by a wide-range of cytotoxic stimulators, in particular, those that denature proteins. However, the HSP70B genes do not respond to DNA damage. We used a PCR array to detect marker genes of DNA damage-related cytotoxic stimulation and found the BTG2 gene to be one such gene. Analysis of the BTG2 gene functional promoter region by transfection of various deletion constructs into HepG2 cells indicated that the p53 and NFY biding sites on BTG2 are important for the response to DNA damage. We then constructed HepG2 sensor cells using the functional BTG2 promoter, and found that these sensor cells can specifically detect the cytotoxicity accompanied by DNA strand breaks with high sensitivity.

本文言語英語
ページ(範囲)1460-1465
ページ数6
ジャーナルBiotechnology and Bioengineering
102
5
DOI
出版ステータス出版済み - 4 1 2009
外部発表はい

All Science Journal Classification (ASJC) codes

  • バイオテクノロジー
  • バイオエンジニアリング
  • 応用微生物学とバイオテクノロジー

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