DNA detection microfluidic device based on negative dielectrophoresis of DNA labeled microbeads

Zhenhao Ding, Kenichi Ida, Kenya Matsuda, Michihiko Nakano, Junya Suehiro

研究成果: 著書/レポートタイプへの貢献会議での発言

抄録

In this study, we focus on the alteration of negative dielectrophoresis (n-DEP) caused by small amount of DNA labeling on the microbeads and propose a new design of microfluidic device for more sensitive DNA detection. In previous study, we proposed a new electrical technique for rapid detection of DNA amplified by polymerase chain reaction (PCR). The method is based on dramatic alteration of dielectrophoresis (DEP) characteristics of microbeads caused by DNA labeling. The DNA labeling of microbeads alters their DEP behavior in such a way that they are trapped on a microelectrode under the action of positive DEP (p-DEP), whereas bare microbeads are not, due to the n-DEP. However, this method requires at least 105 copies of DNA labelling on a microbead to alter the DEP behavior of microbeads from negative to positive. As a result, we are able to distinguish bare microbeads and microbeads labeled with DNA at DNA to microbead ratio of 3×103: 1 and 3×104: 1, based on the n-DEP alteration.

元の言語英語
ホスト出版物のタイトルIEEE SENSORS 2017 - Conference Proceedings
出版者Institute of Electrical and Electronics Engineers Inc.
ページ1-3
ページ数3
2017-December
ISBN(電子版)9781509010127
DOI
出版物ステータス出版済み - 12 21 2017
イベント16th IEEE SENSORS Conference, ICSENS 2017 - Glasgow, 英国
継続期間: 10 30 201711 1 2017

その他

その他16th IEEE SENSORS Conference, ICSENS 2017
英国
Glasgow
期間10/30/1711/1/17

Fingerprint

Electrophoresis
Microfluidics
DNA
Labeling
Microelectrodes
Polymerase chain reaction

All Science Journal Classification (ASJC) codes

  • Electrical and Electronic Engineering

これを引用

Ding, Z., Ida, K., Matsuda, K., Nakano, M., & Suehiro, J. (2017). DNA detection microfluidic device based on negative dielectrophoresis of DNA labeled microbeads. : IEEE SENSORS 2017 - Conference Proceedings (巻 2017-December, pp. 1-3). Institute of Electrical and Electronics Engineers Inc.. https://doi.org/10.1109/ICSENS.2017.8234345

DNA detection microfluidic device based on negative dielectrophoresis of DNA labeled microbeads. / Ding, Zhenhao; Ida, Kenichi; Matsuda, Kenya; Nakano, Michihiko; Suehiro, Junya.

IEEE SENSORS 2017 - Conference Proceedings. 巻 2017-December Institute of Electrical and Electronics Engineers Inc., 2017. p. 1-3.

研究成果: 著書/レポートタイプへの貢献会議での発言

Ding, Z, Ida, K, Matsuda, K, Nakano, M & Suehiro, J 2017, DNA detection microfluidic device based on negative dielectrophoresis of DNA labeled microbeads. : IEEE SENSORS 2017 - Conference Proceedings. 巻. 2017-December, Institute of Electrical and Electronics Engineers Inc., pp. 1-3, 16th IEEE SENSORS Conference, ICSENS 2017, Glasgow, 英国, 10/30/17. https://doi.org/10.1109/ICSENS.2017.8234345
Ding Z, Ida K, Matsuda K, Nakano M, Suehiro J. DNA detection microfluidic device based on negative dielectrophoresis of DNA labeled microbeads. : IEEE SENSORS 2017 - Conference Proceedings. 巻 2017-December. Institute of Electrical and Electronics Engineers Inc. 2017. p. 1-3 https://doi.org/10.1109/ICSENS.2017.8234345
Ding, Zhenhao ; Ida, Kenichi ; Matsuda, Kenya ; Nakano, Michihiko ; Suehiro, Junya. / DNA detection microfluidic device based on negative dielectrophoresis of DNA labeled microbeads. IEEE SENSORS 2017 - Conference Proceedings. 巻 2017-December Institute of Electrical and Electronics Engineers Inc., 2017. pp. 1-3
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