DNA detection microfluidic device based on negative dielectrophoresis of DNA labeled microbeads

Zhenhao Ding, Kenichi Ida, Kenya Matsuda, Michihiko Nakano, Junya Suehiro

研究成果: Chapter in Book/Report/Conference proceedingConference contribution

抄録

In this study, we focus on the alteration of negative dielectrophoresis (n-DEP) caused by small amount of DNA labeling on the microbeads and propose a new design of microfluidic device for more sensitive DNA detection. In previous study, we proposed a new electrical technique for rapid detection of DNA amplified by polymerase chain reaction (PCR). The method is based on dramatic alteration of dielectrophoresis (DEP) characteristics of microbeads caused by DNA labeling. The DNA labeling of microbeads alters their DEP behavior in such a way that they are trapped on a microelectrode under the action of positive DEP (p-DEP), whereas bare microbeads are not, due to the n-DEP. However, this method requires at least 105 copies of DNA labelling on a microbead to alter the DEP behavior of microbeads from negative to positive. As a result, we are able to distinguish bare microbeads and microbeads labeled with DNA at DNA to microbead ratio of 3×103: 1 and 3×104: 1, based on the n-DEP alteration.

本文言語英語
ホスト出版物のタイトルIEEE SENSORS 2017 - Conference Proceedings
出版社Institute of Electrical and Electronics Engineers Inc.
ページ1-3
ページ数3
2017-December
ISBN(電子版)9781509010127
DOI
出版ステータス出版済み - 12 21 2017
イベント16th IEEE SENSORS Conference, ICSENS 2017 - Glasgow, 英国
継続期間: 10 30 201711 1 2017

その他

その他16th IEEE SENSORS Conference, ICSENS 2017
Country英国
CityGlasgow
Period10/30/1711/1/17

All Science Journal Classification (ASJC) codes

  • Electrical and Electronic Engineering

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