DNA Detection using Microbeads-based Dielectrophoretic Impedance Measurement

Michihiko Nakano, Zhenhao Ding, Hiromichi Kasahara, Junya Suehiro

研究成果: ジャーナルへの寄稿Conference article

3 引用 (Scopus)

抄録

A new electrical technique for rapid detection of DNA amplified by polymerase chain reaction (PCR) using dielectrophoresis (DEP) of microbeads was demonstrated. The amplicons were chemically immobilized on the microbeads. The DNA immobilization on the microbeads alters their DEP behavior in such a way that they are trapped on a microelectrode under the action of positive DEP, whereas pristine microbeads are not. Combining the dramatic alteration in DEP characteristics with impedance measurement leads to rapid and quantitative detection of the amplicon in a few seconds. The proposed microbead-based assay could be applicable for rapid, quantitative, and automated detection of virus infection.

元の言語英語
記事番号6985174
ページ(範囲)1010-1013
ページ数4
ジャーナルProceedings of IEEE Sensors
2014-December
発行部数December
DOI
出版物ステータス出版済み - 12 12 2014
イベント13th IEEE SENSORS Conference, SENSORS 2014 - Valencia, スペイン
継続期間: 11 2 201411 5 2014

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Electrophoresis
DNA
immobilization
polymerase chain reaction
Microelectrodes
Polymerase chain reaction
virus
Viruses
assay
Assays
detection

All Science Journal Classification (ASJC) codes

  • Electrical and Electronic Engineering

これを引用

DNA Detection using Microbeads-based Dielectrophoretic Impedance Measurement. / Nakano, Michihiko; Ding, Zhenhao; Kasahara, Hiromichi; Suehiro, Junya.

:: Proceedings of IEEE Sensors, 巻 2014-December, 番号 December, 6985174, 12.12.2014, p. 1010-1013.

研究成果: ジャーナルへの寄稿Conference article

Nakano, Michihiko ; Ding, Zhenhao ; Kasahara, Hiromichi ; Suehiro, Junya. / DNA Detection using Microbeads-based Dielectrophoretic Impedance Measurement. :: Proceedings of IEEE Sensors. 2014 ; 巻 2014-December, 番号 December. pp. 1010-1013.
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