DNA hybridization in reverse micelles and its application to mutation detection

Lian Chun Park, Tatsuo Maruyama, Masahiro Goto

    研究成果: ジャーナルへの寄稿学術誌査読

    18 被引用数 (Scopus)

    抄録

    DNA hybridization was investigated in AOT (sodium di-2-ethylhexyl sulfosuccinate)/isooctane reverse micelles. The single-stranded DNA molecules were encapsulated in the nanoscale water pools formed in the reverse micelles, reducing the hybridization rate. The DNA hybridization can be monitored by simply measuring the UV absorbance of the reverse micellar solution at 260 nm. We found that the DNA hybridization occurred only at the restricted water content (Wo = [H2O]/[AOT] = 20) and below room temperature. We applied this DNA hybridization behavior in reverse micelles to mutation detection in a model gene p53 and successfully detected the single nucleotide mutations in 20-mer, 30-mer and 50-mer nucleotides without a DNA labeling.

    本文言語英語
    ページ(範囲)161-165
    ページ数5
    ジャーナルAnalyst
    128
    2
    DOI
    出版ステータス出版済み - 2月 1 2003

    !!!All Science Journal Classification (ASJC) codes

    • 分析化学
    • 生化学
    • 環境化学
    • 分光学
    • 電気化学

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