DNA manipulation and retrieval from an aqueous solution with micromachined nanotweezers

Gen Hashiguchi, Takushi Goda, Maho Hosogi, Ken Hirano, Noritada Kaji, Yoshinobu Baba, Kuniyuki Kakushima, Hiroyuki Fujita

研究成果: Contribution to journalArticle査読

81 被引用数 (Scopus)

抄録

We have demonstrated DNA handling with micromachined nanotweezers that consist of a pair of opposing nanoprobes and integrated thermal expansion microactuators for changing the probe gap. The probe tips coated with a thin Al layer were dipped into a droplet of a solution containing λ-DNA molecules labeled with fluorescence dye, and then an ac electric field was applied between probes for several seconds. DNA molecules were then captured between the probe tips and retrieved from the solution to the air. The DNA capture between the probe tips could be performed more successfully on the droplet surface than in the underwater region. We also conducted an observation of the retrieved DNA molecules by transmission electron microscope and found that the thickness of the retrieved DNA molecules under the condition of this experiment was ∼21 nm when the time of the applied ac power (1 MHz, 20 Vpp) was 20 s.

本文言語英語
ページ(範囲)4347-4350
ページ数4
ジャーナルAnalytical Chemistry
75
17
DOI
出版ステータス出版済み - 9 1 2003
外部発表はい

All Science Journal Classification (ASJC) codes

  • 分析化学

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