抄録
The germ line reprogramming barrier resets parental epigenetic modifications according to sex, conferring totipotency to mammalian embryos upon fertilization. However, it is not known whether epigenetic errors are committed during germ line reprogramming that are then transmitted to germ cells, and consequently to offspring. We addressed this question in the present study by performing a genome-wide DNA methylation analysis using a target postbisulfite sequencing method in order to identify DNA methylation errors in cloned mouse sperm. The sperm genomes of two somatic cell-cloned mice (CL1 and CL7) contained significantly higher numbers of differentially methylated CpG sites (P1/40.0045 and P1/40.0116). As a result, they had higher numbers of differentially methylated CpG islands. However, there was no evidence that these sites were transmitted to the sperm genome of offspring. These results suggest that DNA methylation errors resulting from embryo cloning are transmitted to the sperm genome by evading the germ line reprogramming barrier.
| 元の言語 | 英語 |
|---|---|
| 記事番号 | 128 |
| ジャーナル | Biology of Reproduction |
| 巻 | 94 |
| 発行部数 | 6 |
| DOI | |
| 出版物ステータス | 出版済み - 6 1 2016 |
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All Science Journal Classification (ASJC) codes
- Cell Biology
これを引用
DNA methylation errors in cloned mouse sperm by germ line barrier evasion. / Koike, Tasuku; Wakai, Takuya; Jincho, Yuko; Sakashita, Akihiko; Kobayashi, Hisato; Mizutani, Eiji; Wakayama, Sayaka; Miura, Fumihito; Ito, Takashi; Kono, Tomohiro.
：: Biology of Reproduction, 巻 94, 番号 6, 128, 01.06.2016.研究成果: ジャーナルへの寄稿 › 記事
}
TY - JOUR
T1 - DNA methylation errors in cloned mouse sperm by germ line barrier evasion
AU - Koike, Tasuku
AU - Wakai, Takuya
AU - Jincho, Yuko
AU - Sakashita, Akihiko
AU - Kobayashi, Hisato
AU - Mizutani, Eiji
AU - Wakayama, Sayaka
AU - Miura, Fumihito
AU - Ito, Takashi
AU - Kono, Tomohiro
PY - 2016/6/1
Y1 - 2016/6/1
N2 - The germ line reprogramming barrier resets parental epigenetic modifications according to sex, conferring totipotency to mammalian embryos upon fertilization. However, it is not known whether epigenetic errors are committed during germ line reprogramming that are then transmitted to germ cells, and consequently to offspring. We addressed this question in the present study by performing a genome-wide DNA methylation analysis using a target postbisulfite sequencing method in order to identify DNA methylation errors in cloned mouse sperm. The sperm genomes of two somatic cell-cloned mice (CL1 and CL7) contained significantly higher numbers of differentially methylated CpG sites (P1/40.0045 and P1/40.0116). As a result, they had higher numbers of differentially methylated CpG islands. However, there was no evidence that these sites were transmitted to the sperm genome of offspring. These results suggest that DNA methylation errors resulting from embryo cloning are transmitted to the sperm genome by evading the germ line reprogramming barrier.
AB - The germ line reprogramming barrier resets parental epigenetic modifications according to sex, conferring totipotency to mammalian embryos upon fertilization. However, it is not known whether epigenetic errors are committed during germ line reprogramming that are then transmitted to germ cells, and consequently to offspring. We addressed this question in the present study by performing a genome-wide DNA methylation analysis using a target postbisulfite sequencing method in order to identify DNA methylation errors in cloned mouse sperm. The sperm genomes of two somatic cell-cloned mice (CL1 and CL7) contained significantly higher numbers of differentially methylated CpG sites (P1/40.0045 and P1/40.0116). As a result, they had higher numbers of differentially methylated CpG islands. However, there was no evidence that these sites were transmitted to the sperm genome of offspring. These results suggest that DNA methylation errors resulting from embryo cloning are transmitted to the sperm genome by evading the germ line reprogramming barrier.
UR - http://www.scopus.com/inward/record.url?scp=84978818962&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84978818962&partnerID=8YFLogxK
U2 - 10.1095/biolreprod.116.138677
DO - 10.1095/biolreprod.116.138677
M3 - Article
C2 - 27103445
AN - SCOPUS:84978818962
VL - 94
JO - Biology of Reproduction
JF - Biology of Reproduction
SN - 0006-3363
IS - 6
M1 - 128
ER -