DNA Methylome Analysis Identifies Transcription Factor-Based Epigenomic Signatures of Multilineage Competence in Neural Stem/Progenitor Cells

Tsukasa Sanosaka, Takuya Imamura, Nobuhiko Hamazaki, Muh Chyi Chai, Katsuhide Igarashi, Maky Ideta-Otsuka, Fumihito Miura, Takashi Ito, Nobuyuki Fujii, Kazuho Ikeo, Kinichi Nakashima

研究成果: ジャーナルへの寄稿記事

10 引用 (Scopus)

抄録

Regulation of the epigenome during in vivo specification of brain stem cells is still poorly understood. Here, we report DNA methylome analyses of directly sampled cortical neural stem and progenitor cells (NS/PCs) at different development stages, as well as those of terminally differentiated cortical neurons, astrocytes, and oligodendrocytes. We found that sequential specification of cortical NS/PCs is regulated by two successive waves of demethylation at early and late development stages, which are responsible for the establishment of neuron- and glia-specific low-methylated regions (LMRs), respectively. The regulatory role of demethylation of the gliogenic genes was substantiated by the enrichment of nuclear factor I (NFI)-binding sites. We provide evidence that de novo DNA methylation of neuron-specific LMRs establishes glia-specific epigenotypes, essentially by silencing neuronal genes. Our data highlight the in vivo implications of DNA methylation dynamics in shaping epigenomic features that confer the differentiation potential of NS/PCs sequentially during development.

元の言語英語
ページ(範囲)2992-3003
ページ数12
ジャーナルCell Reports
20
発行部数12
DOI
出版物ステータス出版済み - 9 19 2017

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Neural Stem Cells
Stem cells
Epigenomics
Mental Competency
Neurons
Transcription Factors
Stem Cells
DNA
DNA Methylation
Genes
NFI Transcription Factors
Neuroglia
Specifications
Brain
Oligodendroglia
Gene Silencing
Binding Sites
Astrocytes
Brain Stem

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)

これを引用

DNA Methylome Analysis Identifies Transcription Factor-Based Epigenomic Signatures of Multilineage Competence in Neural Stem/Progenitor Cells. / Sanosaka, Tsukasa; Imamura, Takuya; Hamazaki, Nobuhiko; Chai, Muh Chyi; Igarashi, Katsuhide; Ideta-Otsuka, Maky; Miura, Fumihito; Ito, Takashi; Fujii, Nobuyuki; Ikeo, Kazuho; Nakashima, Kinichi.

:: Cell Reports, 巻 20, 番号 12, 19.09.2017, p. 2992-3003.

研究成果: ジャーナルへの寄稿記事

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abstract = "Regulation of the epigenome during in vivo specification of brain stem cells is still poorly understood. Here, we report DNA methylome analyses of directly sampled cortical neural stem and progenitor cells (NS/PCs) at different development stages, as well as those of terminally differentiated cortical neurons, astrocytes, and oligodendrocytes. We found that sequential specification of cortical NS/PCs is regulated by two successive waves of demethylation at early and late development stages, which are responsible for the establishment of neuron- and glia-specific low-methylated regions (LMRs), respectively. The regulatory role of demethylation of the gliogenic genes was substantiated by the enrichment of nuclear factor I (NFI)-binding sites. We provide evidence that de novo DNA methylation of neuron-specific LMRs establishes glia-specific epigenotypes, essentially by silencing neuronal genes. Our data highlight the in vivo implications of DNA methylation dynamics in shaping epigenomic features that confer the differentiation potential of NS/PCs sequentially during development.",
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