DNA polymerases and DNA ligases

研究成果: 著書/レポートタイプへの貢献

4 引用 (Scopus)

抄録

DNA polymerase and DNA ligase are ubiquitous enzymes that synthesize complementary DNA strands according to the template DNA and ligate breaks in the backbone structure of DNA, respectively, in living organisms. Multiple enzymes have been identified from each organism, and the functional sharing of these enzymes has been investigated for both DNA polymerase and DNA ligase. In addition to their fundamental role in maintaining genome integrity during replication and repair, DNA polymerases and DNA ligases are widely used for genetic engineering techniques, including DNA cloning, dideoxy sequencing, DNA labeling, mutagenesis, and other in vitro DNA manipulations. Thermostable DNA polymerases and DNA ligases are especially important in PCR and LDR/LCR, which are indispensable techniques for gene manipulation. In this chapter, we have summarized the recent developments in DNA polymerases and DNA ligases from thermophilic microbes.

元の言語英語
ホスト出版物のタイトルThermophilic Microbes in Environmental and Industrial Biotechnology
ホスト出版物のサブタイトルBiotechnology of Thermophiles
出版者Springer Netherlands
ページ429-457
ページ数29
ISBN(電子版)9789400758995
ISBN(印刷物)9789400758988
DOI
出版物ステータス出版済み - 1 1 2013

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DNA Ligases
DNA-Directed DNA Polymerase
DNA
Enzymes
Genetic Techniques
DNA Breaks
Genetic Engineering
DNA Sequence Analysis
Mutagenesis
Organism Cloning
Complementary DNA
Genome
Polymerase Chain Reaction
Genes

All Science Journal Classification (ASJC) codes

  • Medicine(all)

これを引用

Ishino, S., & Ishino, Y. (2013). DNA polymerases and DNA ligases. : Thermophilic Microbes in Environmental and Industrial Biotechnology: Biotechnology of Thermophiles (pp. 429-457). Springer Netherlands. https://doi.org/10.1007/978-94-007-5899-5_17

DNA polymerases and DNA ligases. / Ishino, Sonoko; Ishino, Yoshizumi.

Thermophilic Microbes in Environmental and Industrial Biotechnology: Biotechnology of Thermophiles. Springer Netherlands, 2013. p. 429-457.

研究成果: 著書/レポートタイプへの貢献

Ishino, S & Ishino, Y 2013, DNA polymerases and DNA ligases. : Thermophilic Microbes in Environmental and Industrial Biotechnology: Biotechnology of Thermophiles. Springer Netherlands, pp. 429-457. https://doi.org/10.1007/978-94-007-5899-5_17
Ishino S, Ishino Y. DNA polymerases and DNA ligases. : Thermophilic Microbes in Environmental and Industrial Biotechnology: Biotechnology of Thermophiles. Springer Netherlands. 2013. p. 429-457 https://doi.org/10.1007/978-94-007-5899-5_17
Ishino, Sonoko ; Ishino, Yoshizumi. / DNA polymerases and DNA ligases. Thermophilic Microbes in Environmental and Industrial Biotechnology: Biotechnology of Thermophiles. Springer Netherlands, 2013. pp. 429-457
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