DNA replication in nucleus-free xenopus egg extracts

Ronald Lebofsky, Tatsuro Takahashi, Johannes C. Walter

研究成果: Chapter in Book/Report/Conference proceedingChapter

50 被引用数 (Scopus)

抄録

Extracts derived from Xenopus laevis eggs represent a powerful cell-free system to study eukaryotic DNA replication. A variation of the system allows for DNA replication not only in a cell-free environment, but also in the absence of a nucleus. In this nucleus-free system, DNA templates are licensed with High-Speed Supernatant (HSS) and then replicated with a concentrated NucleoPlasmic Extract (NPE). This method has the advantage of allowing replication of small plasmids with desired modifications and manipulation of the nuclear environment. This chapter describes the protocols needed to prepare HSS and NPE and how these extracts are used to study DNA replication.

本文言語英語
ホスト出版物のタイトルDNA Replication
ホスト出版物のサブタイトルMethods and Protocols
編集者Sonya Vengrova, Jacob Dalgaard
ページ229-252
ページ数24
DOI
出版ステータス出版済み - 12 1 2009
外部発表はい

出版物シリーズ

名前Methods in Molecular Biology
521
ISSN(印刷版)1064-3745

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics

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