DNA sensors using a ferrocene-oligonucleotide conjugate

Masamichi Nakayama, Toshihiro Ihara, Koji Nakano, Mizuo Maeda

    研究成果: Contribution to journalArticle査読

    89 被引用数 (Scopus)

    抄録

    Using the redox-active DNA conjugate (ferrocene-modified oligonucleotide 12 mer) as a probe, the novel electrochemical gene sensor, which is sensitive, convenient, and not relying on radio isotope, has been developed. Oligonucleotide (16 met) complementary to the target (19 met) was immobilized onto gold electrode through the specific chemisorption of successive phosphorothioates which were introduced into 5′-end of the oligonucleotide. The sequence of the conjugate was designed to be also complementary to another site of the target. Therefore, the conjugate and the oligonucleotide anchoring on the electrode formed a sandwich-type ternary complex with a target DNA to give electric currents based on the ferrocene oxidation. By using this system, we have distinguished the mutant that has one base substitution from the fully complementary target.

    本文言語英語
    ページ(範囲)857-866
    ページ数10
    ジャーナルTalanta
    56
    5
    DOI
    出版ステータス出版済み - 4 1 2002

    All Science Journal Classification (ASJC) codes

    • Analytical Chemistry

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