DNA separation by cholesterol-bearing pullulan nanogels

Keisuke Kondo, Noritada Kaji, Sayaka Toita, Yukihiro Okamoto, Manabu Tokeshi, Kazunari Akiyoshi, Yoshinobu Baba

研究成果: Contribution to journalArticle査読

5 被引用数 (Scopus)

抄録

We present an application of a novel DNA separation matrix, cholesterol-bearing pullulan (CHP) nanogels, for microchip electrophoresis. The solution of the CHP showed a unique phase transition around 30 mg/ml and formed gel phase over this critical concentration. This gel phase consists of the weak hydrophobic interactions between the cholesterols could be easily deformed by external forces, and thus, loading process of the CHP nanogels into microchannels became easier. The high concentration of the CHP nanogels provided excellent resolutions especially for small DNA fragments from 100 to 1500 bp. The separation mechanism was discussed based on Ogston and Reptation models which had developed in gels or polymer solutions. The result of a single molecule imaging gave us an insight of the separation mechanism and the nanogel structures as well.

本文言語英語
論文番号032210
ジャーナルBiomicrofluidics
4
3
DOI
出版ステータス出版済み - 2010
外部発表はい

All Science Journal Classification (ASJC) codes

  • 生体医工学
  • 材料科学(全般)
  • 凝縮系物理学
  • 流体および伝熱
  • コロイド化学および表面化学

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