DNA separation in nanowall array chips

Takao Yasui, Noritada Kaji, Ryo Ogawa, Shingi Hashioka, Manabu Tokeshi, Yasuhiro Horiike, Yoshinobu Baba

研究成果: Contribution to journalArticle査読

61 被引用数 (Scopus)

抄録

A nanowall array structure was fabricated on a quartz chip as a separation matrix of DNA fragments, and a 30 s separation was realized for a mixture of DNA fragments (48.5 and 1 kbp fragments) by applying the electric voltage. A longer DNA fragment migrates faster than a shorter one in a nanowall array chip, and it is completely different from the separation of DNA based on gel electrophoresis, nanopillar chips, and nanoparticle array chips. Although the result is similar to DNA separation by entropic trapping, it could not be fully explained by entropic trapping phenomena. Direct observation of single-DNA molecular dynamics inside a nanowall array structure indicates that both confined elongation and relaxation recoiling of a DNA molecule occur, and an elongated DNA molecule migrates faster than a recoiled DNA molecule. Numerical fitting of DNA molecular dynamics reveals that the balance between times for the transverse of a DNA molecule in the nanowall array chip and the relaxation-recoiling of a DNA molecule governs the separation of DNA.

本文言語英語
ページ(範囲)6635-6640
ページ数6
ジャーナルAnalytical Chemistry
83
17
DOI
出版ステータス出版済み - 9 1 2011
外部発表はい

All Science Journal Classification (ASJC) codes

  • 分析化学

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