TY - JOUR
T1 - DNA typing of HLA class II genes in Japanese patients with dilated cardiomyopathy
AU - Nishi, Hirofumi
AU - Koga, Yoshinori
AU - Koyanagi, Takeshi
AU - Harada, Haruhito
AU - Imaizumi, Tsutomu
AU - Toshima, Hironori
AU - Sasazuki, Takehiko
AU - Kimura, Akinori
N1 - Funding Information:
We thank Ors K. Harumi, C. Kawai, K. Kawamura, R. Kusukawa, M. Nagano, Y. Nimura, R. Okada, T. Sugimoto, H. Tanaka, H. Yasuda and Y. Yazaki for providing blood samples from patients with DCM. We also than Dr R. P. Dong for his contributions to the HLA-DNA typing. This work was supported in part by grants-in-aid (05253215 and 05670642) from the Ministry of Education, Culture and Science, Japan, a grant-in-aid (06274105) for scientific research on priority areas, from the Ministry of Education, Culture and Science, Japan, research grants from the Ministry of Health and Welfare, Japan, and research grants from Kirnura Memorial Heart Foundation and Naito Foundation.
PY - 1995/10
Y1 - 1995/10
N2 - HLA class II genes (DRB, DQA, DQB, DPA, and DPB) were typed at the DNA level using polymerase chain reaction/sequence-specfic oligonnucleotide probe analysis in 78 unrelated patients with DCM and 336 unrelated healthy controls to elucidate the HLA alleles or HLA haplotypes associated with DCM. The frequencies of DRB * 1401 (14.4% v 4.5%, RR = 3.90, P<0.0005, Pc<0.03), DQB1*0503 (14.1% v 5.4%, RR=2.93, P<0.007) and DRB1*1401-DQB1*0503 haplotype (11.5% v 1.5%, RR = 8.24, P<0.00001, Pc<0.01) were increased in the DCM patients. The frequency of HLA-DRB1*1101 (9.0% v 3.0%, RR = 3.26, P<0.02) also was increased in the patients. In addition, the frequencies of DQB1*0604 and DPB1*0401 were increased in the DRB1*1401 and DRB1*1101 negative patients. In contrast, the frequencies of DQB1*0303 (19.2% v 30.7%, RR = 0.55, P<0.05) and DRB1*0901-DQB1*0.03 haplotype (16.7% v 29.8%, RR = 0.49, P<0.02) were decreased in the DCM group. Disease susceptibility to DCM in the Japanese population, thus, may be controlled in part by a gene (or genes) in close linkage disequilibrium with DRB1*1401-DQB1*0503, DRB1*1101-DQB1*0301, and DQB1*0604-DPB1*0401 haplotypes, while the resistance to DCM may be associated with the DRB1*0901-DQB1*0303 haplotype.
AB - HLA class II genes (DRB, DQA, DQB, DPA, and DPB) were typed at the DNA level using polymerase chain reaction/sequence-specfic oligonnucleotide probe analysis in 78 unrelated patients with DCM and 336 unrelated healthy controls to elucidate the HLA alleles or HLA haplotypes associated with DCM. The frequencies of DRB * 1401 (14.4% v 4.5%, RR = 3.90, P<0.0005, Pc<0.03), DQB1*0503 (14.1% v 5.4%, RR=2.93, P<0.007) and DRB1*1401-DQB1*0503 haplotype (11.5% v 1.5%, RR = 8.24, P<0.00001, Pc<0.01) were increased in the DCM patients. The frequency of HLA-DRB1*1101 (9.0% v 3.0%, RR = 3.26, P<0.02) also was increased in the patients. In addition, the frequencies of DQB1*0604 and DPB1*0401 were increased in the DRB1*1401 and DRB1*1101 negative patients. In contrast, the frequencies of DQB1*0303 (19.2% v 30.7%, RR = 0.55, P<0.05) and DRB1*0901-DQB1*0.03 haplotype (16.7% v 29.8%, RR = 0.49, P<0.02) were decreased in the DCM group. Disease susceptibility to DCM in the Japanese population, thus, may be controlled in part by a gene (or genes) in close linkage disequilibrium with DRB1*1401-DQB1*0503, DRB1*1101-DQB1*0301, and DQB1*0604-DPB1*0401 haplotypes, while the resistance to DCM may be associated with the DRB1*0901-DQB1*0303 haplotype.
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U2 - 10.1016/S0022-2828(95)92091-9
DO - 10.1016/S0022-2828(95)92091-9
M3 - Article
C2 - 8576952
AN - SCOPUS:0028791482
VL - 27
SP - 2385
EP - 2392
JO - Journal of Molecular and Cellular Cardiology
JF - Journal of Molecular and Cellular Cardiology
SN - 0022-2828
IS - 10
ER -