DNMT3L promotes quiescence in postnatal spermatogonial progenitor cells

Hung Fu Liao, Wendy S.C. Chen, Yu Hsiang Chen, Tzu Hao Kao, Yen Tzu Tseng, Chien Yueh Lee, Yu Chiao Chiu, Pei Lung Lee, Qian Jia Lin, Yung Hao Ching, Kenichiro Hata, Winston T.K. Cheng, Mong Hsun Tsai, Hiroyuki Sasaki, Hong Nerng Ho, Shinn Chih Wu, Yen Hua Huang, Pauline Yen, Shau Ping Lin

    研究成果: Contribution to journalArticle査読

    34 被引用数 (Scopus)

    抄録

    The ability of adult stem cells to reside in a quiescent state is crucial for preventing premature exhaustion of the stem cell pool. However, the intrinsic epigenetic factors that regulate spermatogonial stem cell quiescence are largely unknown. Here, we investigate in mice how DNA methyltransferase 3-like (DNMT3L), an epigenetic regulator important for interpreting chromatin context and facilitating de novo DNA methylation, sustains the long-term male germ cell pool. We demonstrated that stem cell-enriched THY1+ spermatogonial stem/ progenitor cells (SPCs) constituted a DNMT3L-expressing population in postnatal testes. DNMT3L influenced the stability of promyelocytic leukemia zinc finger (PLZF), potentially by downregulating Cdk2/CDK2 expression, which sequestered CDK2-mediated PLZF degradation. Reduced PLZF in Dnmt3l KO THY1+ cells released its antagonist, Sallike protein 4A (SALL4A), which is associated with overactivated ERK and AKT signaling cascades. Furthermore, DNMT3L was required to suppressthe cell proliferation-promoting factor SALL4B in THY1+ SPCs and to prevent premature stem cell exhaustion. Our results indicate that DNMT3L is required to delicately balance the cycling and quiescence of SPCs. These findings reveal a novel role for DNMT3L in modulating postnatal SPC cell fate decisions.

    本文言語英語
    ページ(範囲)2402-2413
    ページ数12
    ジャーナルDevelopment (Cambridge)
    141
    12
    DOI
    出版ステータス出版済み - 2014

    All Science Journal Classification (ASJC) codes

    • 分子生物学
    • 発生生物学

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