Does the hydrolysis of inositol phospholipids lead to the opening of voltage operated Ca2+ channels in guinea-pig ileum? Studies with fluoride ions and caffeine

Stephen P. Watson, Alison F. Stanley, Toshiyuki Sasaguri

研究成果: Contribution to journalArticle査読

15 被引用数 (Scopus)

抄録

Fluoride ions (1 - 30 mM) stimulate phosphoinositide hydrolysis in guinea-pig ileum longitudinal smooth muscle slices, and this is not inhibited in the presence of indomethacin or nifedipine. This action is associated with a slow contractile response which peaks after approximately five minutes and then declines towards baseline; at this time the contractile response to a maximally effecive concentration of carbachol is also inhibited. Fluoride-induced contractions are inhibited completely in the presence of nifedipine. Similarly, contractions induced by caffeine, which releases Ca2+ from intracellular stores, are also inhibited by nifedipine. These data are consistent with a model in which the activation of a G-protein by F- ions leads to the following sequential events: activation of phospholipase C, release of intracellular Ca2+ opening of voltage operated (i.e. dihydropyridine sensitive) Ca2+ channels and contraction. The transient nature of the fluoride contraction and the inhibition of the carbachol contraction may be due to a slow elevation of cAMP levels induced by F-.

本文言語英語
ページ(範囲)14-20
ページ数7
ジャーナルBiochemical and Biophysical Research Communications
153
1
DOI
出版ステータス出版済み - 5 31 1988
外部発表はい

All Science Journal Classification (ASJC) codes

  • 生物理学
  • 生化学
  • 分子生物学
  • 細胞生物学

フィンガープリント

「Does the hydrolysis of inositol phospholipids lead to the opening of voltage operated Ca<sup>2+</sup> channels in guinea-pig ileum? Studies with fluoride ions and caffeine」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル