Down-regulation of AMP-activated protein kinase by calorie restriction in rat liver

Kazuo To, Haruyoshi Yamaza, Toshimitsu Komatsu, Takahiro Hayashida, Hiroko Hayashi, Hiroaki Toyama, Takuya Chiba, Yoshikazu Higami, Isao Shimokawa

研究成果: ジャーナルへの寄稿学術誌査読

37 被引用数 (Scopus)


AMP-activated protein kinase (AMPK) may act as a key enzyme for metabolic adaptation to calorie restriction (CR) or reduced growth hormone (GH)-insulin-like growth factor (IGF)-1 signaling, an experimental intervention for lifespan extension in animals. We investigated the protein levels of AMPKα and a downstream enzyme, acetyl-CoA carboxylase (ACC), by immunoblotting of liver and quadriceps femoris muscle (QFM) extracts from 6-month-old wild-type (W) and GH-suppressed transgenic (Tg) Wistar rats fed ad libitum (AL) or 30% CR diets from 6 weeks of age. A modified alternate-day feeding regimen for CR yielded a fed-fasted cycle in CR rats, and therefore the effects of overnight fasting in W-AL rats were also evaluated. CR decreased threonine-172-phosphorylated AMPKα (p-AMPKα; an activated form) levels in the liver, whereas the CR-fed-fasted cycle or overnight fasting did not significantly affect the p-AMPKα level. In the QFM, the p-AMPKα level was slightly elevated in the CR-fasted phase, but greatly increased in the AL-fasted phase. Suppression of GH did not affect the p-AMPKα level. The phosphorylated-ACC levels did not alter in parallel with the p-AMPKα level, particularly in the liver. The present results suggest that CR down-regulates the AMPK activity in the liver on a long-term basis.

ジャーナルExperimental Gerontology
出版ステータス出版済み - 11月 2007

!!!All Science Journal Classification (ASJC) codes

  • 生化学
  • 加齢科学
  • 分子生物学
  • 遺伝学
  • 内分泌学
  • 細胞生物学


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