Droplet-based microfluidic sensing system for rapid fish freshness determination

Daisuke Itoh, Fumihiro Sassa, Taiji Nishi, Yoko Kani, Masakazu Murata, Hiroaki Suzuki

研究成果: Contribution to journalArticle査読

27 被引用数 (Scopus)

抄録

A microfluidic device was constructed for on-site determination of fish freshness by using adenosine-5′-triphosphate (ATP) concentration as an indicator of freshness. In the device, extracts from fish and reagent solutions were processed in the form of droplets separated by air. The volumes of the droplets of the extract solution, a solution containing enzyme substrates, and a washing buffer solution were measured in branched flow channels. To simplify the structure and procedure for mixing solutions, a wider portion was formed in one flow channel to merge the droplets. A row of droplets was ejected from each of three branched flow channels, merged in the wider portion, and transported to a sensing region located downstream. ATP was detected using two enzymatic reactions involving glycerol kinase (GK) and glycerol-3-phosphate oxidase (G3PO). A linear relationship between the generated current and ATP concentration was confirmed, and ATP detection from jack mackerel extracts was carried out. A distinct change in the current was observed with the fresh extracts as in the case of standard solutions. The obtained data correlated well with those obtained by high-performance liquid chromatography (HPLC).

本文言語英語
ページ(範囲)619-626
ページ数8
ジャーナルSensors and Actuators, B: Chemical
171-172
DOI
出版ステータス出版済み - 8 2012
外部発表はい

All Science Journal Classification (ASJC) codes

  • 電子材料、光学材料、および磁性材料
  • 器械工学
  • 凝縮系物理学
  • 表面、皮膜および薄膜
  • 金属および合金
  • 電子工学および電気工学
  • 材料化学

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