Dual-immunohistochemistry provides little evidence for epithelial- mesenchymal transition in pulmonary fibrosis

Mizuho Yamada, Kazuyoshi Kuwano, Takashige Maeyama, Naoki Hamada, Michihiro Yoshimi, Yoichi Nakanishi, Michael Kasper

研究成果: ジャーナルへの寄稿学術誌査読

56 被引用数 (Scopus)

抄録

epithelial-mesenchymal transition (EMT) has been considered to be involved in organ fibrogenesis. However, there is few direct evidence of this process in the pathophysiology of pulmonary fibrosis in vivo. Therefore, we tried to verify the involvement of this process in the development of pulmonary fibrosis. Since the co-expressions of epithelial and mesenchymal markers are thought to be a marker of EMT, we performed dual-immuunohistochemistry to assess the co-expressions of these proteins in lung tissues from bleomycin-induced pulmonary fibrosis in mice, and from patients with idiopathic pulmonary fibrosis, and nonspecific interstitial pneumonia. Double positive cells for epithelial markers including E-cadherin, T1α, or aquaporin 5, and a mesenchymal markers α-smooth muscle actin or vimentin were not found in bleomycin-induced pulmonary fibrosis in mice. Double positive cells for E-cadherin, ICAM-1, LEA, CD44v9, or SP-A and α-smooth muscle actin or vimentin were not found in lung tissues from normal lung parenchyma, idiopathic pulmonary fibrosis and nonspecific interstitial pneumonia. These results offer at least two possibilities. One is that EMT does not occur in IPF or bleomycin-induced pulmonary fibrosis in mice. Another is that EMT may occur in pulmonary fibrosis but the time during this transition in which cells express detectable levels of epithelial and mesenchymal markers is too small to be detected by double immunohistochemistry.

本文言語英語
ページ(範囲)453-462
ページ数10
ジャーナルHistochemistry and Cell Biology
129
4
DOI
出版ステータス出版済み - 4月 2008

All Science Journal Classification (ASJC) codes

  • 組織学
  • 分子生物学
  • 医療検査技術
  • 細胞生物学

フィンガープリント

「Dual-immunohistochemistry provides little evidence for epithelial- mesenchymal transition in pulmonary fibrosis」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル