Dual regulatory effects of interferon-α, -β, and - γ on interleukin-8 gene expression by human gingival fibroblasts in culture upon stimulation with lipopolysaccharide from Prevotella intermedia, interleukin-1α, or tumor necrosis factor-α

T. Sakuta, M. Tokuda, M. Tamura, E. Jimi, T. Ikebe, T. Koga, S. Nagaoka, H. Takada

研究成果: ジャーナルへの寄稿学術誌査読

28 被引用数 (Scopus)

抄録

In a previous study, we demonstrated that the amount of interleukin (IL)-8 mRNA expressed by human gingival fibroblasts stimulated with lipopolysaccharide (LPS) from Prevotella intermedia ATCC 25611 is increased by pre-treatment with beta or gamma interferon (IFN-β or -γ). In the present study, we identified the regulatory effects of these IFNs on IL-8 mRNA expression and IL-8 production by human gingival fibroblasts. Priming with IFN-alpha (α), -β, or -γ upregulated the IL-8 mRNA expression in response to P. intermedia LPS, whereas co-stimulation with these IFNs reduced the amount of mRNA expressed by the cells. The regulation of IL-8 mRNA expression induced by recombinant human tumor necrosis factor-α (rHuTNF-α) or rHuIL-1α was similar to that induced by LPS. The IL-8 mRNA expression in response to P. intermedia LPS was enhanced by IFN-γ independently of de novo protein synthesis, and was regulated, at least in part, at the transcriptional level. The IL-8 mRNA accumulation in response to P. intermedia LPS was inhibited by tosylphenyl-alanyl chloromethyl-ketone, an inhibitor of NF-κB activation, although the NF-κB activation itself was not altered by IFN-γ. These findings suggest that IFNs might be capable of both enhancing and inhibiting inflammatory responses in periodontal tissues through the dual regulation of IL-8 production by gingival fibroblasts in response to bacterial components and cytokines.

本文言語英語
ページ(範囲)1597-1605
ページ数9
ジャーナルJournal of Dental Research
77
8
DOI
出版ステータス出版済み - 1998
外部発表はい

!!!All Science Journal Classification (ASJC) codes

  • 歯科学(全般)

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