抄録
Approximately 1 percent of healthy individuals carry human herpesvirus-6 within a host chromosome. This is referred to as chromosomally integrated herpesvirus-6 (CIHHV-6). In this study, we investigated the chromosomal integration site in six individuals harboring CIHHV-6B. Using FISH, we found that HHV-6B signals are consistently located at the telomeric region. The proximal endpoints of the integrated virus were mapped at one of two telomere-repeat-like sequences (TRSs) within the DR-R in all cases. In two cases, we isolated junction fragments between the viral TRS and human telomere repeats. The distal endpoints were mapped at the distal TRS in all cases. The size of the distal TRS was found to be ~5 kb which is sufficient to fulfill cellular telomeric functions. We conclude that the viral TRS in the DR regions fulfill dual functions for CIHHV-6: homology-mediated integration into the telomeric region of the chromosome and neo-telomere formation that is then stably transmitted.
| 元の言語 | 英語 |
|---|---|
| 記事番号 | 4559 |
| ジャーナル | Scientific reports |
| 巻 | 4 |
| DOI | |
| 出版物ステータス | 出版済み - 4 2 2014 |
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All Science Journal Classification (ASJC) codes
- General
これを引用
Dual roles for the telomeric repeats in chromosomally integrated human herpesvirus-6. / Ohye, Tamae; Inagaki, Hidehito; Ihira, Masaru; Higashimoto, Yuki; Kato, Koji; Oikawa, Junko; Yagasaki, Hiroshi; Niizuma, Takahiro; Takahashi, Yoshiyuki; Kojima, Seiji; Yoshikawa, Tetsushi; Kurahashi, Hiroki.
：: Scientific reports, 巻 4, 4559, 02.04.2014.研究成果: ジャーナルへの寄稿 › 記事
}
TY - JOUR
T1 - Dual roles for the telomeric repeats in chromosomally integrated human herpesvirus-6
AU - Ohye, Tamae
AU - Inagaki, Hidehito
AU - Ihira, Masaru
AU - Higashimoto, Yuki
AU - Kato, Koji
AU - Oikawa, Junko
AU - Yagasaki, Hiroshi
AU - Niizuma, Takahiro
AU - Takahashi, Yoshiyuki
AU - Kojima, Seiji
AU - Yoshikawa, Tetsushi
AU - Kurahashi, Hiroki
PY - 2014/4/2
Y1 - 2014/4/2
N2 - Approximately 1 percent of healthy individuals carry human herpesvirus-6 within a host chromosome. This is referred to as chromosomally integrated herpesvirus-6 (CIHHV-6). In this study, we investigated the chromosomal integration site in six individuals harboring CIHHV-6B. Using FISH, we found that HHV-6B signals are consistently located at the telomeric region. The proximal endpoints of the integrated virus were mapped at one of two telomere-repeat-like sequences (TRSs) within the DR-R in all cases. In two cases, we isolated junction fragments between the viral TRS and human telomere repeats. The distal endpoints were mapped at the distal TRS in all cases. The size of the distal TRS was found to be ~5 kb which is sufficient to fulfill cellular telomeric functions. We conclude that the viral TRS in the DR regions fulfill dual functions for CIHHV-6: homology-mediated integration into the telomeric region of the chromosome and neo-telomere formation that is then stably transmitted.
AB - Approximately 1 percent of healthy individuals carry human herpesvirus-6 within a host chromosome. This is referred to as chromosomally integrated herpesvirus-6 (CIHHV-6). In this study, we investigated the chromosomal integration site in six individuals harboring CIHHV-6B. Using FISH, we found that HHV-6B signals are consistently located at the telomeric region. The proximal endpoints of the integrated virus were mapped at one of two telomere-repeat-like sequences (TRSs) within the DR-R in all cases. In two cases, we isolated junction fragments between the viral TRS and human telomere repeats. The distal endpoints were mapped at the distal TRS in all cases. The size of the distal TRS was found to be ~5 kb which is sufficient to fulfill cellular telomeric functions. We conclude that the viral TRS in the DR regions fulfill dual functions for CIHHV-6: homology-mediated integration into the telomeric region of the chromosome and neo-telomere formation that is then stably transmitted.
UR - http://www.scopus.com/inward/record.url?scp=84897552295&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84897552295&partnerID=8YFLogxK
U2 - 10.1038/srep04559
DO - 10.1038/srep04559
M3 - Article
C2 - 24691081
AN - SCOPUS:84897552295
VL - 4
JO - Scientific Reports
JF - Scientific Reports
SN - 2045-2322
M1 - 4559
ER -