Duplication and loss of function of genes encoding RNA polymerase III subunit C4 causes hybrid incompatibility in rice

Giao Ngoc Nguyen, Yoshiyuki Yamagata, Yuko Shigematsu, Miyako Watanabe, Yuta Miyazaki, Kazuyuki Doi, Kosuke Tashiro, Satoru Kuhara, Hiroyuki Kanamori, Jianzhong Wu, Takashi Matsumoto, Hideshi Yasui, Atsushi Yoshimura

研究成果: ジャーナルへの寄稿記事

10 引用 (Scopus)

抄録

Reproductive barriers are commonly observed in both animals and plants, in which they maintain species integrity and contribute to speciation. This report shows that a combination of loss-offunction alleles at two duplicated loci, DUPLICATED GAMETOPHYTIC STERILITY 1 (DGS1) on chromosome 4 and DGS2 on chromosome 7, causes pollen sterility in hybrid progeny derived from an interspecific cross between cultivated rice, Oryza sativa, and an Asian annual wild rice, O. nivara. Male gametes carrying the DGS1 allele from O. nivara (DGS1-nivara s ) and the DGS2 allele from O. sativa (DGS2-T65 s ) were sterile, but female gametes carrying the same genotype were fertile. We isolated the causal gene, which encodes a protein homologous to DNA-dependent RNA polymerase (RNAP) III subunit C4 (RPC4). RPC4 facilitates the transcription of 5S rRNAs and tRNAs. The loss-of-function alleles at DGS1-nivara s and DGS2-T65 s were caused by weak or nonexpression of RPC4 and an absence of RPC4, respectively. Phylogenetic analysis demonstrated that gene duplication of RPC4 at DGS1 and DGS2 was a recent event that occurred after divergence of the ancestral population of Oryza from other Poaceae or during diversification of AA-genome species.

元の言語英語
ページ(範囲)2565-2575
ページ数11
ジャーナルG3: Genes, Genomes, Genetics
7
発行部数8
DOI
出版物ステータス出版済み - 1 1 2017

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RNA Polymerase III
Infertility
Alleles
Genes
Germ Cells
Chromosomes, Human, Pair 4
Chromosomes, Human, Pair 7
Gene Duplication
Pollen
Transfer RNA
Poaceae
Oryza
Genotype
Genome
Population

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics
  • Genetics(clinical)

これを引用

Nguyen, G. N., Yamagata, Y., Shigematsu, Y., Watanabe, M., Miyazaki, Y., Doi, K., ... Yoshimura, A. (2017). Duplication and loss of function of genes encoding RNA polymerase III subunit C4 causes hybrid incompatibility in rice. G3: Genes, Genomes, Genetics, 7(8), 2565-2575. https://doi.org/10.1534/g3.117.043943

Duplication and loss of function of genes encoding RNA polymerase III subunit C4 causes hybrid incompatibility in rice. / Nguyen, Giao Ngoc; Yamagata, Yoshiyuki; Shigematsu, Yuko; Watanabe, Miyako; Miyazaki, Yuta; Doi, Kazuyuki; Tashiro, Kosuke; Kuhara, Satoru; Kanamori, Hiroyuki; Wu, Jianzhong; Matsumoto, Takashi; Yasui, Hideshi; Yoshimura, Atsushi.

:: G3: Genes, Genomes, Genetics, 巻 7, 番号 8, 01.01.2017, p. 2565-2575.

研究成果: ジャーナルへの寄稿記事

Nguyen, GN, Yamagata, Y, Shigematsu, Y, Watanabe, M, Miyazaki, Y, Doi, K, Tashiro, K, Kuhara, S, Kanamori, H, Wu, J, Matsumoto, T, Yasui, H & Yoshimura, A 2017, 'Duplication and loss of function of genes encoding RNA polymerase III subunit C4 causes hybrid incompatibility in rice', G3: Genes, Genomes, Genetics, 巻. 7, 番号 8, pp. 2565-2575. https://doi.org/10.1534/g3.117.043943
Nguyen, Giao Ngoc ; Yamagata, Yoshiyuki ; Shigematsu, Yuko ; Watanabe, Miyako ; Miyazaki, Yuta ; Doi, Kazuyuki ; Tashiro, Kosuke ; Kuhara, Satoru ; Kanamori, Hiroyuki ; Wu, Jianzhong ; Matsumoto, Takashi ; Yasui, Hideshi ; Yoshimura, Atsushi. / Duplication and loss of function of genes encoding RNA polymerase III subunit C4 causes hybrid incompatibility in rice. :: G3: Genes, Genomes, Genetics. 2017 ; 巻 7, 番号 8. pp. 2565-2575.
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AU - Nguyen, Giao Ngoc

AU - Yamagata, Yoshiyuki

AU - Shigematsu, Yuko

AU - Watanabe, Miyako

AU - Miyazaki, Yuta

AU - Doi, Kazuyuki

AU - Tashiro, Kosuke

AU - Kuhara, Satoru

AU - Kanamori, Hiroyuki

AU - Wu, Jianzhong

AU - Matsumoto, Takashi

AU - Yasui, Hideshi

AU - Yoshimura, Atsushi

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