Duplication of the psbA gene in the chloroplast genome of two Pinus species

Jonas Lidholm, Alfred Szmidt, Petter Gustafsson

研究成果: Contribution to journalArticle査読

28 被引用数 (Scopus)

抄録

The psbA gene, encoding the D1 protein of photosystem II, was found to be duplicated in the chloroplast genome of two pine species, Pinus contorta and P. banksiana. Analysis of cloned overlapping restriction fragments of P. contorta chloroplast DNA showed that the two psbA genes have the same orientation and are separated by approximately 3.3 kb. The nucleotide sequences of the coding and the upstream regions of the two psbA copies were found to be identical, whereas the downstream sequences diverged from a point 20 by 3′ of the stop codons. Downstream of the gene copy designated psbAII, a dyad symmetry which allows the formation of a strong mRNA hairpin structure, and a trnH gene were found. No such elements, which are characteristic of psbA downstream regions, were found 3′ of psbAI. This suggests that psbAII is the ancestral gene copy in P. contorta. Upon comparison with psbA from other plants, the pine 353-codnn sequence appeared almost as distant from the angiosperm as from the liverwort counterpart. As compared to tobacco, 14 substitutions in the predicted amino acid sequence were found, most of which were located in the terminal regions of the protein.

本文言語英語
ページ(範囲)345-352
ページ数8
ジャーナルMolecular and General Genetics
226
3
DOI
出版ステータス出版済み - 5 1991
外部発表はい

All Science Journal Classification (ASJC) codes

  • 遺伝学

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