DWARF4 accumulation in root tips is enhanced via blue light perception by cryptochromes

Jun Sakaguchi, Tomonao Matsushita, Yuichiro Watanabe

研究成果: ジャーナルへの寄稿記事

抄録

Brassinosteroid (BR) signalling is known to be coordinated with light signalling in above ground tissue. Many studies focusing on the shade avoidance response in above ground tissue or hypocotyl elongation in darkness have revealed the contribution of the BR signalling pathway to these processes. We previously analysed the expression of DWARF 4 (DWF4), a key BR biosynthesis enzyme, and revealed that light perception in above ground tissues triggered DWF4 accumulation in root tips. To determine the required wavelength of light and photoreceptors responsible for this regulation, we studied DWF4-GUS marker plants grown in several monochromatic light conditions. We revealed that monochromatic blue LED light could induce DWF4 accumulation in primary root tips and root growth as much as white light, whereas monochromatic red LED could not. Consistent with this, a cryptochrome1/2 double mutant showed retarded root growth under white light whereas a phytochromeA/B double mutant did not. Taken together, our data strongly indicated that blue light signalling was important for DWF4 accumulation in root tips and root growth. Furthermore, DWF4 accumulation patterns in primary root tips were not altered by auxin or sugar treatment. Therefore, we hypothesize that blue light signalling from the shoot tissue is different from auxin and sugar signalling.

元の言語英語
ページ(範囲)1615-1629
ページ数15
ジャーナルPlant Cell and Environment
42
発行部数5
DOI
出版物ステータス出版済み - 5 1 2019

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Cryptochromes
Meristem
blue light
root tips
brassinosteroids
Light
root growth
white light
Brassinosteroids
auxins
sugars
mutants
Indoleacetic Acids
photoreceptors
hypocotyls
wavelengths
shade
biosynthesis
Growth
shoots

All Science Journal Classification (ASJC) codes

  • Physiology
  • Plant Science

これを引用

DWARF4 accumulation in root tips is enhanced via blue light perception by cryptochromes. / Sakaguchi, Jun; Matsushita, Tomonao; Watanabe, Yuichiro.

:: Plant Cell and Environment, 巻 42, 番号 5, 01.05.2019, p. 1615-1629.

研究成果: ジャーナルへの寄稿記事

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abstract = "Brassinosteroid (BR) signalling is known to be coordinated with light signalling in above ground tissue. Many studies focusing on the shade avoidance response in above ground tissue or hypocotyl elongation in darkness have revealed the contribution of the BR signalling pathway to these processes. We previously analysed the expression of DWARF 4 (DWF4), a key BR biosynthesis enzyme, and revealed that light perception in above ground tissues triggered DWF4 accumulation in root tips. To determine the required wavelength of light and photoreceptors responsible for this regulation, we studied DWF4-GUS marker plants grown in several monochromatic light conditions. We revealed that monochromatic blue LED light could induce DWF4 accumulation in primary root tips and root growth as much as white light, whereas monochromatic red LED could not. Consistent with this, a cryptochrome1/2 double mutant showed retarded root growth under white light whereas a phytochromeA/B double mutant did not. Taken together, our data strongly indicated that blue light signalling was important for DWF4 accumulation in root tips and root growth. Furthermore, DWF4 accumulation patterns in primary root tips were not altered by auxin or sugar treatment. Therefore, we hypothesize that blue light signalling from the shoot tissue is different from auxin and sugar signalling.",
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