TY - JOUR
T1 - Dynamic and coordinated expression profile of dbl-family guanine nucleotide exchange factors in the developing mouse brain
AU - Yoshizawa, Masato
AU - Sone, Masaki
AU - Matsuo, Naoki
AU - Nagase, Takahiro
AU - Ohara, Osamu
AU - Nabeshima, Yo Ichi
AU - Hoshino, Mikio
N1 - Funding Information:
We thank Y. Shimazu and H Yoshimura for aid in computer analyses, R. Yu for helpful comments on the manuscript, the Kyoto University Radioisotope Research Center for allowing us to use the image scanner, and T. Obata for technical assistance. This work was supported by Grant-in-Aid for Scientific Research on Priority Areas (C) Advanced Brain Science Project, (C) Genome Science and (A) Research for Comprehensive Promotion of Study of Brain (to M. H.) from the Ministry of Education, Culture, Sports, Science and Technology of Japan. M. S. was a research fellow of the Japan Society for the Promotion of Science.
PY - 2003/6
Y1 - 2003/6
N2 - Dbl-family guanine nucleotide exchange factors (Dbl-GEFs) act as activators of Rho-like small G proteins such as Rac1, Cdc42 and RhoA. Recently, some GEFs have been suggested to play important roles in the development of the nervous system. Here, we report a comprehensive expression profile analysis of 20 Dbl-GEFs that have yet to be well investigated. Northern analyses of murine mRNAs from brains of E13, E17, P7 and adult mice revealed expression of 18 out of 20 GEFs in some or all stages. In addition, we found that three human GEFs were highly expressed in the brain. Examination of the spatial expression patterns of five GEFs in embryos or neonatal brain by in situ hybridization revealed distinct patterns for each GEF. Our study reveals the dynamic and coordinated expression profiles of the Dbl-GEFs and provides a basic framework for understanding the function of GEFs in neural development.
AB - Dbl-family guanine nucleotide exchange factors (Dbl-GEFs) act as activators of Rho-like small G proteins such as Rac1, Cdc42 and RhoA. Recently, some GEFs have been suggested to play important roles in the development of the nervous system. Here, we report a comprehensive expression profile analysis of 20 Dbl-GEFs that have yet to be well investigated. Northern analyses of murine mRNAs from brains of E13, E17, P7 and adult mice revealed expression of 18 out of 20 GEFs in some or all stages. In addition, we found that three human GEFs were highly expressed in the brain. Examination of the spatial expression patterns of five GEFs in embryos or neonatal brain by in situ hybridization revealed distinct patterns for each GEF. Our study reveals the dynamic and coordinated expression profiles of the Dbl-GEFs and provides a basic framework for understanding the function of GEFs in neural development.
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U2 - 10.1016/S1567-133X(03)00002-4
DO - 10.1016/S1567-133X(03)00002-4
M3 - Article
C2 - 12799088
AN - SCOPUS:0037604662
SN - 1567-133X
VL - 3
SP - 375
EP - 381
JO - Gene Expression Patterns
JF - Gene Expression Patterns
IS - 3
ER -