Dynamic bacterial community changes in the autothermal thermophilic aerobic digestion process with cell lysis activities, shaking and temperature increase

Huijun Cheng, Yuya Asakura, Kosuke Kanda, Ryo Fukui, Yoshihisa Kawano, Yuki Okugawa, Yukihiro Tashiro, Kenji Sakai

研究成果: ジャーナルへの寄稿記事

1 引用 (Scopus)

抄録

Autothermal thermophilic aerobic digestion (ATAD) is conducted for stabilization of sludge waste and is driven by the action of various microorganisms under aerobic conditions. However, the mechanism controlling bacterial community changes during ATAD via three (initial, middle and final) phases is currently unclear. To investigate this mechanism, activity analysis and a microcosm assay with shaking were performed on a bacterial community during the initial, middle, and final phases of incubation. Cell lysis activities toward gram-negative bacteria, but not gram-positive bacteria, were detected in the ATAD samples in the middle and final phases. During shaking incubation in initial-phase samples at 30 °C, major operational taxonomic units (OTUs) related to Acinetobacter indicus and Arcobacter cibarius dramatically increased along with decreases in several major OTUs. In middle-phase samples at 45 °C, we observed a major alteration of OTUs related to Caldicellulosiruptor bescii and Aciditerrimonas ferrireducens, together with distinct decreases in several other OTUs. Final-phase samples maintained a stable bacterial community with major OTUs showing limited similarities to Heliorestis baculata, Caldicellulosiruptor bescii, and Ornatilinea apprima. In conclusion, the changes in the bacterial community observed during ATAD could be partially attributed to the cell lysis activity toward gram-negative bacteria in the middle and final phases. The microcosm assay suggested that certain physical factors, such as a high oxygen supply and shearing forces, also might contribute to bacterial community changes in the initial and middle phases, and to the stable bacterial community in the final phase of ATAD.

元の言語英語
ページ(範囲)196-204
ページ数9
ジャーナルJournal of Bioscience and Bioengineering
126
発行部数2
DOI
出版物ステータス出版済み - 8 1 2018

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Digestion
Bacteria
Temperature
Assays
Oxygen supply
Gram-Negative Bacteria
Shearing
Arcobacter
Microorganisms
Stabilization
Acinetobacter
Gram-Positive Bacteria
Sewage
Oxygen

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

これを引用

Dynamic bacterial community changes in the autothermal thermophilic aerobic digestion process with cell lysis activities, shaking and temperature increase. / Cheng, Huijun; Asakura, Yuya; Kanda, Kosuke; Fukui, Ryo; Kawano, Yoshihisa; Okugawa, Yuki; Tashiro, Yukihiro; Sakai, Kenji.

:: Journal of Bioscience and Bioengineering, 巻 126, 番号 2, 01.08.2018, p. 196-204.

研究成果: ジャーナルへの寄稿記事

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abstract = "Autothermal thermophilic aerobic digestion (ATAD) is conducted for stabilization of sludge waste and is driven by the action of various microorganisms under aerobic conditions. However, the mechanism controlling bacterial community changes during ATAD via three (initial, middle and final) phases is currently unclear. To investigate this mechanism, activity analysis and a microcosm assay with shaking were performed on a bacterial community during the initial, middle, and final phases of incubation. Cell lysis activities toward gram-negative bacteria, but not gram-positive bacteria, were detected in the ATAD samples in the middle and final phases. During shaking incubation in initial-phase samples at 30 °C, major operational taxonomic units (OTUs) related to Acinetobacter indicus and Arcobacter cibarius dramatically increased along with decreases in several major OTUs. In middle-phase samples at 45 °C, we observed a major alteration of OTUs related to Caldicellulosiruptor bescii and Aciditerrimonas ferrireducens, together with distinct decreases in several other OTUs. Final-phase samples maintained a stable bacterial community with major OTUs showing limited similarities to Heliorestis baculata, Caldicellulosiruptor bescii, and Ornatilinea apprima. In conclusion, the changes in the bacterial community observed during ATAD could be partially attributed to the cell lysis activity toward gram-negative bacteria in the middle and final phases. The microcosm assay suggested that certain physical factors, such as a high oxygen supply and shearing forces, also might contribute to bacterial community changes in the initial and middle phases, and to the stable bacterial community in the final phase of ATAD.",
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AU - Asakura, Yuya

AU - Kanda, Kosuke

AU - Fukui, Ryo

AU - Kawano, Yoshihisa

AU - Okugawa, Yuki

AU - Tashiro, Yukihiro

AU - Sakai, Kenji

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