Dynamic cross-linking effect of Mg2+ to enhance sieving properties of low-viscosity poly(vinylpyrrolidone) solutions for microchip electrophoresis of proteins

Mohamad Reza Mohamadi, Noritada Kaji, Anabu Tokeshi, Yoshinobu Baba

研究成果: Contribution to journalArticle査読

12 被引用数 (Scopus)

抄録

We report a dynamic cross-linking effect of Mg2+ that enhances the sieving properties of low-viscosity poly-(vinylpyrrolidone) (PVP) solutions. A low-viscosity PVP solution was applied to nondenaturing microchip electrophoresis of protein samples using microchips made of poly(methyl methacrylate). The separation resolution of nondenatured protein markers in 1.8% PVP solution was improved by adding 1-20 mM MgCl2. We studied the effect of the ratio of cross-linking agent on mobility of protein samples and showed that protein retardation (In μ/μ0) is correlated with the ratio of cross-linking agent to PVP ([cMg2+/cPVP]) as In μ/μ0 = A′[cMg2+ /cPVP] b′. A′ was related to the protein radius (R), and b′ was found to be 0.72 for proteins with R = 2.4 nm and 0.82 for proteins with R = 1.85 nm. A structural study of PVP in semidilute solutions using dynamic light scattering showed that incremental increases of Mg2+ ion concentration from 5 to 20 mM in 1.8% PVP solution increased the hydrodynamic radius of PVP polymers by 20%.

本文言語英語
ページ(範囲)312-316
ページ数5
ジャーナルAnalytical Chemistry
80
1
DOI
出版ステータス出版済み - 1 1 2008
外部発表はい

All Science Journal Classification (ASJC) codes

  • 分析化学

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